Identification of multiple cell surface antigens associated with the sex pheromone response of Streptococcus faecalis.
Identification of multiple cell surface antigens associated with the sex pheromone response of Streptococcus faecalis.
复制标题
与粪链球菌性信息素反应相关的多种细胞表面抗原的鉴定。
DOI：
10.1128/jb.162.1.131-137.1985
复制
发表时间：
1985
影响因子：
3.2
通讯作者：
Dunny,GM
中科院分区：
文献类型：
--
作者：
Tortorello,ML;Dunny,GM
高分子量的表面抗原，通过硫酸铵沉淀的培养上清液，并确定在十二烷基硫酸钠-聚丙烯酰胺凝胶的蛋白质印迹，已与粪链球菌供体细胞的性信息素反应。携带接合质粒pCF 10的信息素诱导的细胞产生由130千道尔顿范围内的至少四条带组成的抗原组分（C130）和73千道尔顿抗原（SA 73）。供体细胞暴露于信息素制剂后，培养上清液中C130抗原的浓度随时间增加。凝胶过滤研究表明，这种抗原以天然状态存在，作为一个非常大的复合物，其大小超过180，000道尔顿。C130抗原易被蛋白酶K消化，并且不与伴刀豆球蛋白A或麦胚凝集素反应。C130的抗原性在抗体显色前用胰蛋白酶、糜蛋白酶或木瓜蛋白酶处理印迹未被破坏，而SA 73的抗原性对这些处理敏感。
High-molecular-weight surface antigens, obtained by ammonium sulfate precipitation of culture supernatants and identified in Western blots of sodium dodecyl sulfate-polyacrylamide gels, have been correlated with the sex pheromone response of Streptococcus faecalis donor cells. Pheromone-induced cells carrying the conjugative plasmid pCF10 produced both an antigenic component (C130) composed of at least four bands in the range of 130 kilodaltons and a 73-kilodalton antigen (SA 73). The concentration of the C130 antigen in culture supernatants increased with time after exposure of donor cells to pheromone preparations. Gel filtration studies indicated that this antigen exists in the native state as a very large complex that is more than 180,000 daltons in size. The C130 antigen was susceptible to digestion by proteinase K and was not reactive with either concanavalin A or wheat germ agglutinin. The antigenicity of C130 was not destroyed by treatment of blots with trypsin, chymotrypsin, or papain before development with antibody, whereas the antigenicity of SA73 was susceptible to these treatments.