Mobilization of endothelial progenitor cells in patients with acute myocardial infarction
Mobilization of endothelial progenitor cells in patients with acute myocardial infarction
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DOI：
10.1161/hc2301.092122
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发表时间：
2001-06-12
期刊：
影响因子：
37.8
通讯作者：
Imaizumi, T
中科院分区：
文献类型：
--
作者：
Shintani, S;Murohara, T;Imaizumi, T
背景-内皮祖细胞在成人外周血中循环并促进新生血管形成.然而，关于EPCs及其推定的前体CD 34阳性单核细胞是否与EPCs的表达相关，方法和结果-流式细胞术显示循环MNCCD 34+计数在急性心肌梗塞患者（n=16）中显著增加，在发病后第7天达到峰值，而在没有心脏缺血证据的对照受试者（n=8）中它们没有变化。在培养过程中，PB-MNCs形成多个细胞簇，具有内皮细胞谱系标志物（CD 31、血管内皮钙粘蛋白和激酶插入结构域受体）的EPC样附着细胞从簇中萌发。在急性心肌梗死患者中，从第7天获得的培养的PB-MNCs中产生的细胞簇和EPCs比第1天产生的细胞簇和EPCs多。血管内皮生长因子的血浆水平显著增加，在第7天达到峰值，并且它们与循环MNCCD 34+计数正相关（r=0.35，P=0.01）。结论-这是第一次临床证明，表明谱系承诺的EPCs和MNCCD 34+，它们的推定前体，在人类急性缺血事件期间被动员。
Background - Endothetial progenitor cells (EPCs) circulate in adult peripheral blood (PB) and contribute to neovascularization. However, little is known regarding whether EPCs and their putative precursor, CD34-positive mononuclear cells (MNCCD34+),, mobilized into PB in acute ischemic events in humans.Methods and Results - Flow cytometry revealed that circulating MNCCD34+ counts significantly increased in patients with acute myocardial infarction (n=16), peaking on day 7 after onset, whereas they were unchanged in control subjects (n=8) who had no evidence of cardiac ischemia. During culture, PB-MNCs formed multiple cell clusters, and EPC-like attaching cells with endothelial cell lineage markers (CD31, vascular endothelial cadherin, and kinase insert domain receptor) sprouted from clusters. In patients with acute myocardial infarction, more cell clusters and EPCs developed from cultured PB-MNCs obtained on day 7 than those on day 1. Plasma levels of vascular endothelial growth factor significantly increased, peaking on day 7, and they positively correlated with circulating MNCCD34+ counts (r=0.35, P=0.01).Conclusions - This is the first clinical demonstration showing that lineage-committed EPCs and MNCCD34+, their putative precursors, are mobilized during an acute ischemic event in humans.