Structure characterization of an exopolysaccharide produced by Bifidobacterium animalis RH
Structure characterization of an exopolysaccharide produced by Bifidobacterium animalis RH
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DOI：
10.1016/j.carbpol.2012.08.012
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发表时间：
2013-01-02
影响因子：
11.2
通讯作者：
Li, Pinglan
中科院分区：
文献类型：
--
作者：
Shang, Nan;Xu, Rihua;Li, Pinglan
从百岁老人粪便中分离纯化了动物双歧杆菌RH产生的胞外多糖（EPSb），并对其结构和构象进行了表征。傅里叶变换红外光谱、凝胶渗透色谱、高ph阴离子交换色谱-脉冲安培检测、高酸盐氧化和Smith降解、甲基化和气相色谱-质谱分析以及核磁响应分析结果表明，EPSb （M-w = 21.3 kDa）由鼠李糖（Rha）、阿拉伯糖（Ara）、半乳糖（Gal）、葡萄糖（Glc）和甘露糖（Man）组成，摩尔比为0.4:0.3:1.6:0.8:1.2。该化合物具有(1 - >,4)-连接Man、(1 - bbb4)-连接Rha、(1 - bbb4)-连接Gal和(1 - bbb4)-连接Glc的骨架。它的分支是Gal末端是Gal和Glc残基。通过原子力显微镜观察了EPSb的分子结构。EPSb显示球形块状物和环状网络。构象分析揭示了EPSb的非三螺旋构象。(C) 2012 Elsevier Ltd.版权所有。
An exopolysaccharide fraction (EPSb) produced by Bifidobacterium animalis RH isolated from the feces of centenarians was purified to illustrate its structure and conformational characterization. Results from Fourier-transform infrared spectrometry, gel permeation chromatography, high-pH anion-exchange chromatography with pulsed amperometric detection, periodate oxidation and Smith degradation, methylation and gas chromatography-mass spectrometry, and nuclear magnetic response analysis indicated that EPSb (M-w = 21.3 kDa) was composed of rhamnose (Rha), arabinose (Ara), galactose (Gal), glucose (Glc), and mannose (Man) in a molar ratio of 0.4:0.3:1.6:0.8:1.2. This compound had a backbone of (1 -> 3,4)-linked Man, (1 -> 4)-linked Rha, (1 -> 4)-linked Gal, and (1 -> 4)-linked Glc. It was branched with Gal and terminated with Gal and Glc residues. The molecular structure of EPSb was observed via atomic force microscopy. EPSb showed spherical lumps and a ring-like network. Conformational analysis revealed the non-triple helical conformation of EPSb. (C) 2012 Elsevier Ltd. All rights reserved.