DIF-1, putative morphogen of D-discoideum, suppresses cell growth and promotes retinoic acid-induced cell differentiation in HL-60
DIF-1, putative morphogen of D-discoideum, suppresses cell growth and promotes retinoic acid-induced cell differentiation in HL-60
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DOI：
10.1006/bbrc.1997.6964
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发表时间：
1997-07-18
影响因子：
3.1
通讯作者：
Kubohara, Y
中科院分区：
文献类型：
--
作者：
Kubohara, Y
分化诱导因子-1（DIF-1）是一种诱导盘基网柄菌柄细胞形成的形态发生因子。本研究观察了DIF-1对人白血病HL-60细胞的作用。10-40 μ M的DIF-1以剂量依赖性方式抑制细胞生长，15-20 μ M的DIF-1达到约50%的生长抑制。使用碘化丙啶进行的细胞周期时相分布的FAGS分析显示，用15-20 μ M DIF-1处理后，许多细胞聚集在G1期。DIF-1的这些浓度也以剂量依赖性方式升高[Ca 2 +]（i），而与细胞外Ca 2+的存在无关，表明DIF-1引起一些细胞内Ca 2+库的Ca 2+释放。最重要的是，发现相对低浓度的DIF-1（1-5 μ M）促进视黄酸诱导的细胞分化。本结果表明，DIF-1可能是用于分析髓样细胞分化的有用工具，并且在治疗人髓样白血病中具有治疗潜力。(C)1997年学术出版社。
Differentiation-inducing factor-1 (DIF-1) is a putative morphogen that induces stalk cell formation in the cellular slime mold Dictyostelium discoideum. In this study, we have examined the effects of DIF-1 on the human leukemia HL-60 cells. DIF-1 at 10-40 mu M suppressed cell growth in a dose-dependent manner, and approximately 50% growth inhibition was attained with 15-20 mu M DIF-1. FAGS analysis of cell-cycle phase distribution using propidium iodide revealed that many cells were accumulated in the G1 phase after treatment with 15-20 mu M DIF-1. These concentrations of DIF-1 also raised [Ca2+](i) in a dose-dependent manner irrespective of the presence of extracellular Ca2+, indicating that DIF-1 elicited Ca2+-release from some intracellular Ca2+ store(s). Most importantly, relatively low concentrations of DIF-1 (1-5 mu M) were found to promote retinoic acid-induced cell differentiation, The present results indicate that DIF-1 may be a useful tool for the analysis of myeloid cell differentiation and have therapeutic potential in the treatment of human myeloid leukemia. (C) 1997 Academic Press.