Rootcap structure in wild type and in a starchless mutant of Arabidopsis.
Rootcap structure in wild type and in a starchless mutant of Arabidopsis.
复制标题
野生型和拟南芥无淀粉突变体的根冠结构。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
J. Kiss
中科院分区：
文献类型：
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作者：
F. Sack;J. Kiss
拟南芥野生型（WT）和无淀粉向重力性突变体（TC 7）的根冠。通过电子显微镜检查，以确定相对于重力的细胞极性。在柱细胞中，细胞核位于近端，核被膜与内质网（ER）相连，内质网又与附近的胞间连丝相连。浸渍的ER与锇铁氰化物揭示了许多接触之间的柱状质体和ER在两种基因型。ER主要存在于柱原生质体的外部区域，除了正在发育成外周细胞的较老的柱细胞。在垂直根中，只有发育中间的柱细胞（层2细胞）在细胞的远端比近端区域具有更高的ER表面密度（S）。ER的远端S而不是近端S在整个鼻小柱发育过程中是恒定的。与WT的那些相比，质体在TC7柱细胞中较少沉淀。据推测，质体接触ER在两种基因型的重力感知中起作用。
Rootcaps of the wild type (WT) and of a starchless, gravitropic mutant (TC7) of Arabidopsis thaliana L. were examined by electron microscopy to identify cellular polarities with respect to gravity. In columella cells, nuclei are located proximally, and the nuclear envelope is continuous with endoplasmic reticulum (ER) that is in turn connected to nearby plasmodesmata. Impregnation of ER with osmium ferricyanide revealed numerous contacts between columella plastids and ER in both genotypes. ER is present mostly in the outer regions of the columella protoplast except in older columella cells that are developing into peripheral cells. In vertical roots, only columella cells that are intermediate in development (story 2 cells) have a higher surface density (S) of ER in the distal compared to proximal regions of the cell. The distal but not the proximal S of the ER is constant throughout columella development. Plastids are less sedimented in TC7 columella cells compared to those of the WT. It is hypothesized that plastid contact with the ER plays a role in gravity perception in both genotypes.