Evolution of a pathogen: a comparative genomics analysis identifies a genetic pathway to pathogenesis in Acinetobacter.
Evolution of a pathogen: a comparative genomics analysis identifies a genetic pathway to pathogenesis in Acinetobacter.
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病原体的进化：比较基因组学分析确定了不动杆菌发病机制的遗传途径。
DOI：
10.1371/journal.pone.0054287
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Keim P
中科院分区：
文献类型：
--
作者：
Sahl JW;Gillece JD;Schupp JM;Waddell VG;Driebe EM;Engelthaler DM;Keim P
鲍曼不动杆菌是一种全球性的医院感染病原菌。除A.除了鲍曼不动杆菌外，其他不动杆菌属物种，特别是醋酸钙不动杆菌-鲍曼不动杆菌（Acb）复合体中的那些，也与严重的人类感染有关。尽管对A.鲍曼不动杆菌已经被鉴定出来，解释鲍曼不动杆菌出现的遗传机制。鲍曼不动杆菌作为分布最广且毒性最强的不动杆菌种尚未完全了解。最近的全基因组测序提供了深入了解不动杆菌属的系统发育结构。然而，不动杆菌属之间的基因组特征的全球比较。在文献中没有描述。在这项研究中，136个不动杆菌基因组，包括67个测序在这项研究中，进行了比较，以确定在不动杆菌属的扩张基因的获得和丢失。全基因组测序证实A. baumannii是一个单系分支，较大的Acb复合体也是一个得到充分支持的单系群。全基因组同源性提供了一个框架，全球基因组比较的基础上，爆炸评分比（BSR）分析。BSR分析表明，在进化过程中，特定的基因既有丢失的，也有获得的。鲍曼不动杆菌。此外，还研究了与A.鲍曼不动杆菌Acb复合体的致病机制更为保守，尤其是在A.鲍曼不动杆菌，比其他不动杆菌的基因组;直到最近，全球的分布和保护的毒力因子的分析是不可能的。结果表明，获得特定的毒力因子可能有助于广泛的持久性和毒力的A。鲍曼不动杆菌。识别与转录调控相关的新功能，并通过Acb复合体中的进化枝获得，为更好地了解该属扩展中的致病性和毒力的演变提供了目标。
Acinetobacter baumannii is an emergent and global nosocomial pathogen. In addition to A. baumannii, other Acinetobacter species, especially those in the Acinetobacter calcoaceticus-baumannii (Acb) complex, have also been associated with serious human infection. Although mechanisms of attachment, persistence on abiotic surfaces, and pathogenesis in A. baumannii have been identified, the genetic mechanisms that explain the emergence of A. baumannii as the most widespread and virulent Acinetobacter species are not fully understood. Recent whole genome sequencing has provided insight into the phylogenetic structure of the genus Acinetobacter. However, a global comparison of genomic features between Acinetobacter spp. has not been described in the literature. In this study, 136 Acinetobacter genomes, including 67 sequenced in this study, were compared to identify the acquisition and loss of genes in the expansion of the Acinetobacter genus. A whole genome phylogeny confirmed that A. baumannii is a monophyletic clade and that the larger Acb complex is also a well-supported monophyletic group. The whole genome phylogeny provided the framework for a global genomic comparison based on a blast score ratio (BSR) analysis. The BSR analysis demonstrated that specific genes have been both lost and acquired in the evolution of A. baumannii. In addition, several genes associated with A. baumannii pathogenesis were found to be more conserved in the Acb complex, and especially in A. baumannii, than in other Acinetobacter genomes; until recently, a global analysis of the distribution and conservation of virulence factors across the genus was not possible. The results demonstrate that the acquisition of specific virulence factors has likely contributed to the widespread persistence and virulence of A. baumannii. The identification of novel features associated with transcriptional regulation and acquired by clades in the Acb complex presents targets for better understanding the evolution of pathogenesis and virulence in the expansion of the genus.
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