Central nervous system transcriptome of Biomphalaria alexandrina, an intermediate host for schistosomiasis.
Central nervous system transcriptome of Biomphalaria alexandrina, an intermediate host for schistosomiasis.
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DOI：
10.1186/s13104-017-3018-6
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发表时间：
2017-12-11
影响因子：
1.8
通讯作者：
Miller MW
中科院分区：
文献类型：
--
作者：
Mansour TA;Habib MR;Rodríguez LCV;Vázquez AH;Alers JM;Ghezzi A;Croll RP;Brown CT;Miller MW
在全球范围内，超过2亿人生活在被忽视的热带疾病血吸虫病（或蜗牛热）的风险中。幼虫寄生虫需要特定的蜗牛物种作为中间宿主，支持它们的繁殖和转化为可以感染人类的形式。该项目旨在从埃及曼氏血吸虫的主要中间宿主亚历山大双脐螺的中枢神经系统（CNS）中产生转录组。从从亚历山大B杆菌未感染标本解剖的五个合并的中枢神经系统产生转录组。原始Illumina RNA-seq数据（每个150个碱基对长度的约2030万个配对末端读段）生成了由144，213个转录物元件组成的转录组，其中N50重叠群大小为716个碱基对。在UniProtKB/Swiss-Prot数据库中鉴定了15，246个转录本的直系同源物和另外16，810个转录本的同源物。B。alexandrina CNS转录组为未来探索更简单的神经系统中寄生虫-宿主相互作用的研究提供了资源。此外，对亚历山大B. alexandrina对曼氏血吸虫幼虫感染的反应中所涉及的神经信号机制的理解的增加可能会导致控制蜗牛种群的新的和高度特异性的策略。本文的在线版本（10.1186/s13104-017-3018-6）包含补充材料，可供授权用户使用。
Globally, more than 200 million people live at risk of the neglected tropical disease schistosomiasis (or snail fever). Larval schistosomes require the presence of specific snail species that act as intermediate hosts, supporting their multiplication and transformation into forms that can infect humans. This project was designed to generate a transcriptome from the central nervous system (CNS) of Biomphalaria alexandrina, the major intermediate host for Schistosoma mansoni in Egypt. A transcriptome was generated from five pooled central nervous systems dissected from uninfected specimens of B. alexandrina. Raw Illumina RNA-seq data (~ 20.3 million paired end reads of 150 base pairs length each) generated a transcriptome consisting of 144,213 transcript elements with an N50 contig size of 716 base pairs. Orthologs of 15,246 transcripts and homologs for an additional 16,810 transcripts were identified in the UniProtKB/Swiss-Prot database. The B. alexandrina CNS transcriptome provides a resource for future research exploring parasite-host interactions in a simpler nervous system. Moreover, increased understanding of the neural signaling mechanisms involved in the response of B. alexandrina to infection by S. mansoni larvae could lead to novel and highly specific strategies for the control of snail populations. The online version of this article (10.1186/s13104-017-3018-6) contains supplementary material, which is available to authorized users.