An untargeted metabolomics approach to determine component differences and variation in their in vivo distribution between Kuqin and Ziqin, two commercial specifications of Scutellaria Radix
An untargeted metabolomics approach to determine component differences and variation in their in vivo distribution between Kuqin and Ziqin, two commercial specifications of Scutellaria Radix
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DOI：
10.1039/c7ra10705f
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发表时间：
2017-11
期刊：
影响因子：
3.9
通讯作者：
Zhi-Wei Yang;Feng Xu;Xin Liu;Yi Cao;Qi Tang;Qian-Yu Chen;M. Shang;Guang-xue Liu;Xuan Wang;S. Cai
中科院分区：
文献类型：
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作者：
Zhi-Wei Yang;Feng Xu;Xin Liu;Yi Cao;Qi Tang;Qian-Yu Chen;M. Shang;Guang-xue Liu;Xuan Wang;S. Cai
苦芩（Kuqin， KQ）和紫芩（Ziqin， ZQ）是黄芩的两种重要的商品规格，源自黄芩的根。中医认为，KQ用于治疗上补肺热证，ZQ用于清除下补大肠热证。KQ和ZQ功效差异的化学基础目前尚不清楚。在这里，我们提出了一种非靶向代谢组学方法来快速筛选和鉴定KQ和ZQ之间的化学和体内分布差异。共鉴定出114个成分差异，其中35个为首次在SR中鉴定。此外，口服SR后，在大鼠结肠和肺中初步鉴定出19种原型成分和16种代谢物，其中6种原型成分和12种代谢物为首次报道。观察黄芩苷、枸杞子苷、枸杞子苷和欧oxylin A在结肠和肺部的分布差异。为了解释这种差异，提出了它们的代谢途径。通过提取的离子色谱计算黄芩苷与周周醇的峰面积比，作为黄芩苷与周周醇的鉴别指标，用于黄芩苷与周周醇的分级和质量控制。这些结果可能部分解释了KQ与ZQ的疗效差异，并为相关疾病的临床治疗提供参考。
Kuqin (KQ) and Ziqin (ZQ), derived from the roots of Scutellaria baicalensis Georgi, are two important commercial specifications of Scutellariae Radix (SR, termed Huang qin in Chinese). According to traditional Chinese medicine, KQ is used for the treatment of upper energizer lung heat syndrome while ZQ is used to clear lower energizer large intestine heat syndrome. The chemical basis for differences in efficacy between KQ and ZQ is currently unknown. Here, we present an untargeted metabolomics approach to rapidly screen and identify chemical and in vivo distribution differences between KQ and ZQ. We identified 114 constituent differences between KQ and ZQ, of which 35 were identified in SR for the first time. Furthermore, 19 prototype constituents and 16 metabolites were tentatively identified in rat colon and lungs after oral administration of SR, of which six prototype constituents and 12 metabolites were reported for the first time. Distribution differences of baicalin, wogonoside, wogonin and oroxylin A between colon and lungs were observed. To interpret such differences, their metabolic pathways were proposed. The peak area ratio of baicalin to eriodictyol calculated from the extracted ion chromatogram was proposed as a differentiation index for the classification and quality control of KQ and ZQ. These results may partially explain the efficacy differences between KQ and ZQ, and provide references for clinical treatment of related diseases.