Characterization of a new strain of Taura syndrome virus (TSV) from Colombian shrimp farms and the implication in the selection of TSV resistant lines
Characterization of a new strain of Taura syndrome virus (TSV) from Colombian shrimp farms and the implication in the selection of TSV resistant lines
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DOI：
10.1016/j.jip.2012.08.009
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发表时间：
2013-01-01
影响因子：
3.4
通讯作者：
Lightner, Donald
中科院分区：
文献类型：
--
作者：
Fernando Aranguren, Luis;Salazar, Marcela;Lightner, Donald
在2004年之前，哥伦比亚虾养殖受益于一项选择计划，其中南美白对虾种群被开发为对Taura综合症（TS）具有抗性。然而，自2004年以来，TS作为一种重要疾病再次出现。2010年，在哥伦比亚收集到一种显然是新的TSV菌株（命名为CO 10）。它的基因组测序，并与其他六个完全测序的分离株进行比较。分析表明，CO 10 TSV与来自夏威夷和委内瑞拉的分离株关系密切.基于59个TSV分离物的衣壳蛋白2（CP 2）区域的系统发育分析显示，最近的哥伦比亚分离物（2006-2010）形成一个新的簇，并且与先前的哥伦比亚分离物（1994-1998）在核苷酸序列上相差4%。该CO 10分离株的毒力与通过在凡纳滨对虾中实验感染确定的伯利兹TSV相似，显示100%死亡率和相似的存活曲线。通过TSV的RT-qPCR，来自CO 10和伯利兹的感染虾中的病毒载量也接近，约为1 x 10（10）拷贝/μ 1 RNA。为了开发抗TSV品系，候选虾应该用最近从它们将被养殖的地区分离的病毒株进行攻击。这项研究表明，TSV存在于哥伦比亚虾养殖场在过去的5年中，是一个新的TSV菌株具有高毒力。(C)2012 Elsevier Inc. All rights reserved.
Prior to 2004, Colombian shrimp farming benefited from a selection program in which Penaeus vannamei stocks were developed with resistance to Taura syndrome disease (TS). However since 2004, TS reappeared as a significant disease. In 2010, an apparently new strain of TSV (designated as CO 10) was collected in Colombia. Its genome was sequenced and compared with six other fully sequenced isolates. This analysis revealed that the TSV CO 10 is closely related to the isolates from Hawaii and Venezuela. Phylogenetic analysis based on capsid protein 2 (CP2) region from 59 TSV isolates shows that the recent Colombian isolates (2006-2010) form a new cluster and differ from the previous Colombia isolates (1994-1998) by 4% in nucleotide sequence. The virulence of this CO 10 isolate was similar to a Belize TSV determined through experimental infection in P. vannamei showing 100% mortalities and similar survival curves. By RT-qPCR for TSV, the viral loads were also close in the infected shrimp from both CO 10 and Belize at the order of 1 x 10(10) copies per mu 1 RNA. To develop TSV-resistant lines, the candidate shrimp should be challenged with virus strains that have been isolated most recently from the regions where they will be cultured. This study suggests that the TSV present in Colombian shrimp farms during the last 5 years is a new TSV strain with high virulence. (C) 2012 Elsevier Inc. All rights reserved.