EFFECTS OF RNA SECONDARY STRUCTURE ON ALTERNATIVE SPLICING OF PRE-MESSENGER RNA - IS FOLDING LIMITED TO A REGION BEHIND THE TRANSCRIBING RNA-POLYMERASE
EFFECTS OF RNA SECONDARY STRUCTURE ON ALTERNATIVE SPLICING OF PRE-MESSENGER RNA - IS FOLDING LIMITED TO A REGION BEHIND THE TRANSCRIBING RNA-POLYMERASE
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DOI：
10.1016/0092-8674(88)90202-4
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发表时间：
1988-07-29
期刊：
影响因子：
64.5
通讯作者：
EPERON, IC
中科院分区：
文献类型：
--
作者：
EPERON, LP;GRAHAM, IR;EPERON, IC
隐藏在潜在RNA二级结构的短茎内的替代5''剪接位点在体内的使用由环的长度决定。超过环路长度阈值时，尽管存在潜在结构，仍会使用替代位点。相比之下，在我们测试的所有长度的环的体外剪接过程中，替代位点的使用很少或根本不使用。提出的模型表明前 mRNA 仅在转录后的有限时间内自由折叠。
The use in vivo of an alternative 5'' splice site sequestered within a short stem of potential RNA secondary structure is determined by the length of the loop. Above a threshold length of loop, the alternative site is used despite the potential structure. In contrast, the alternative site is used very little or not at all during splicing in vitro with all lengths of loop that we have tested. A model is proposed which suggests that pre-mRNA is free to fold only within a limit period after transcription.