Cytokine responses to eye spray adjuvants for enhancing vaccine-induced immunity in chickens.
Cytokine responses to eye spray adjuvants for enhancing vaccine-induced immunity in chickens.
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细胞因子对眼喷雾佐剂的反应，用于增强鸡疫苗诱导的免疫力。
DOI：
10.1111/1348-0421.12391
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发表时间：
2016
影响因子：
2.6
通讯作者：
Tsukasa Seya
中科院分区：
文献类型：
--
作者：
Hiromi Takaki;Haruko Sato;Riho Kurata;Hirokazu Hikono;Takahiro Hiono;Hiroshi Kida;Misako Matsumoto;Takehiko Saito;Tsukasa Seya
用于鸡的眼喷雾流感疫苗越来越多;然而，如何增强细胞和抗体对它们的反应仍然没有确定。在此，在鸡中评估了含有免疫增强佐剂Pam 2CSK 4或polyI：C的滴眼液。将这些TLR激动剂应用于鸡结膜导致脾、肺和哈氏腺中IL-1β的上调，而不是其他细胞因子，包括IFN和IL-6。因此，对鸡结膜粘膜应用佐剂的反应不同于哺乳动物鼻内佐剂的预期反应。确定合适的佐剂递送途径对于在鸡中引起免疫应答至关重要。
Eye spray influenza vaccines for chickens are increasingly available; however, how to enhance cellular and antibody responses to them remains undetermined. Here, eye‐drops containing the immune‐enhancing adjuvants Pam2CSK4 or polyI:C were assessed in chickens. Application of these TLR agonists to chicken conjunctiva resulted in up‐regulation of IL‐1β, but not other cytokines, including IFN and IL‐6, in the spleen, lung and Harderian gland. Thus, responses to adjuvant applied to the conjunctival mucosa of chickens differ from those expected from the responses to intra‐nasal adjuvants in mammals. Identifying an appropriate delivery route for adjuvants is crucial for evoking immune responses in chickens.