Assessment of Kidney Function in Mouse Models of Glomerular Disease
Assessment of Kidney Function in Mouse Models of Glomerular Disease
复制标题
肾小球疾病小鼠模型肾功能的评估
DOI：
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复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
S. Oltean
中科院分区：
文献类型：
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作者：
M. Stevens;S. Oltean
使用鼠模型来模拟人类肾脏疾病正变得越来越普遍。我们的研究重点是评估糖尿病肾病和足细胞特异性VEGF-A敲除小鼠的肾小球功能;因此，本方案描述了我们实验室用于评估这些肾小球疾病小鼠模型的完整肾脏检查，从而能够从单个小鼠中获得有关肾脏和肾小球功能的大量信息。与文献中提出的评估肾小球功能的替代方法相比，使用本文概述的方法可以从多个方面全面评估肾小球表型。通过使用这种方法，研究人员可以确定模型的肾脏表型，并评估表型发展的机制。在这些模型中检查潜在的治疗途径时，需要关于疾病机制的重要信息。该方法允许通过测量尿白蛋白肌酐比和个体肾小球水渗透性以及使用过碘酸希夫染色和电子显微镜进行结构和超微结构检查来详细评估肾小球滤过屏障的功能。此外，在mRNA和蛋白质水平上对基因失调的分析使得能够对肾小球功能进行机制分析。该方案概述了通用但适应性强的方法，可应用于所有肾小球疾病的小鼠模型。
The use of murine models to mimic human kidney disease is becoming increasingly common. Our research is focused on the assessment of glomerular function in diabetic nephropathy and podocyte-specific VEGF-A knock-out mice; therefore, this protocol describes the full kidney work-up used in our lab to assess these mouse models of glomerular disease, enabling a vast amount of information regarding kidney and glomerular function to be obtained from a single mouse. In comparison to alternative methods presented in the literature to assess glomerular function, the use of the method outlined in this paper enables the glomerular phenotype to be fully evaluated from multiple aspects. By using this method, the researcher can determine the kidney phenotype of the model and assess the mechanism as to why the phenotype develops. This vital information on the mechanism of disease is required when examining potential therapeutic avenues in these models. The methods allow for detailed functional assessment of the glomerular filtration barrier through measurement of the urinary albumin creatinine ratio and individual glomerular water permeability, as well as both structural and ultra-structural examination using the Periodic Acid Schiff stain and electron microscopy. Furthermore, analysis of the genes dysregulated at the mRNA and protein level enables mechanistic analysis of glomerular function. This protocol outlines the generic but adaptable methods that can be applied to all mouse models of glomerular disease.
DOI：
10.1152/ajprenal.00021.2010
发表时间：
2010-07-01
影响因子：
4.2
作者：
Zhang, Hongyu;Schin, MaryLee;Brosius, Frank C., III
通讯作者：
Brosius, Frank C., III