Inhibition of T cell receptor-coreceptor interactions by antagonist ligands visualized by live FRET imaging of the T-hybridoma immunological synapse.
Inhibition of T cell receptor-coreceptor interactions by antagonist ligands visualized by live FRET imaging of the T-hybridoma immunological synapse.
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DOI：
10.1016/s1074-7613(02)00301-1
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发表时间：
2002-04
期刊：
影响因子：
32.4
通讯作者：
T. Zal;M. Zal;N. Gascoigne
中科院分区：
文献类型：
--
作者：
T. Zal;M. Zal;N. Gascoigne
TCR激动剂和拮抗剂对T细胞活化的不同作用被认为是通过CD 4或CD 8共受体向TCR-MHC 0复合物的差异性募集而改变的。我们使用三维活细胞成像的荧光共振能量转移（FRET）之间的CD 3+和CD 4融合的变体的绿色荧光蛋白研究TCR-CD 4相互作用在T细胞活化。我们证明了激动剂MHCp复合物的识别触发了CD 4和TCR之间的分子间相互作用，可在T-杂交瘤-APC接触区域检测到。这种相互作用被拮抗剂配体的存在所阻断，而不减少CD 4和CD 4的募集或阻止它们在免疫突触中的部分共定位。
The diverse effects of TCR agonists and antagonists on T cell activation are believed to be modified by the differential recruitment of CD4 or CD8 coreceptors to the TCR-MHCp complex. We used three-dimensional live cell imaging of fluorescence resonance energy transfer (FRET) between CD3ζ and CD4 fused to variants of the green fluorescent protein to investigate TCR-CD4 interactions during T cell activation. We demonstrate that recognition of agonist MHCp complexes triggers intermolecular interaction between CD4 and TCR, detectable across the T-hybridoma-APC contact area. This interaction is blocked by the presence of antagonist ligands without decreasing the recruitment of ζ and CD4 or preventing their partial colocalization in the immunological synapse.