Melanocortin-3 Receptors Expressed on Agouti-Related Peptide Neurons Inhibit Feeding Behavior in Female Mice.
Melanocortin-3 Receptors Expressed on Agouti-Related Peptide Neurons Inhibit Feeding Behavior in Female Mice.
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DOI：
10.1002/oby.22306
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Butler AA
中科院分区：
文献类型：
--
作者：
Girardet C;Marks DL;Butler AA
能量不足时下丘脑AgRP +ve神经元的激活是一种负价态信号，快速激活寻食行为。本研究考察了AgRP+ve神经元表达的MC3Rs的作用。通过将AgRP- ires - cre小鼠与在5 ' UTR中含有“loxP-STOP-loxP”序列的LoxTBMc3r小鼠杂交，产生在AgRP+ve神经元中选择性表达MC3Rs的AgRP- mc3r小鼠。在自由摄食和限时摄食两种摄食条件下，对大鼠的体重、体组成和摄食行为进行评估。在雌性小鼠中，agro - ires - cre (+ve) （n=7）或agro - ires - cre (-ve) （n=9）小鼠的食物摄入量无显著差异；因此，这些老鼠被集中起来组成“对照组”。与对照组（35.3±1.8 kJ/12h, n=16）和LoxTBMc3r小鼠（32.1±2.1 kJ/12h, n=9）相比，雌性agro - mc3r小鼠在黑暗期活动期的摄食量（25.4±2.4 kJ/12h, n=6）较低。当小鼠消耗较少的食物（9-10 kJ）时，在休息阶段（灯亮）的食物摄入量在基因型之间是正常的。AgRP-MC3R和LoxTBMc3r小鼠的体重和组成相似，提示热量摄入减少的补偿机制。值得注意的是，在禁食和限时喂养后，AgRP-MC3R小鼠在重新喂食期间继续消耗更少的食物。在AgRP+ve神经元上表达的MC3Rs似乎对控制摄食行为的下丘脑网络施加了强烈的抑制信号。
Activation of hypothalamic agouti-related peptide (AgRP)+ve neurons during energy deficit is a negative valence signal, rapidly activating food seeking behaviors. This study examined the roles of MC3Rs expressed by AgRP+ve neurons. AgRP-MC3R mice expressing MC3Rs selectively in AgRP+ve neurons were generated by crossing AgRP-IRES-Cre mice with LoxTBMc3r mice containing a “loxP-STOP-loxP” sequence in the 5’ UTR. Body weight, body composition and feeding behavior were assessed during ad libitum and timed-restricted feeding conditions. In females, food intake of AgRP-IRES-Cre(+ve) (n=7) or AgRP-IRES-Cre(-ve) (n=9) mice was not significantly different; these mice were therefore pooled to form the “control” group. Female AgRP-MC3R mice exhibited lower food intake (25.4±2.4 kJ/12h, n=6) compared to controls (35.3±1.8 kJ/12h, n=16) and LoxTBMc3r mice (32.1±2.1 kJ/12h, n=9) in the active phase during the dark period. Food intake during the rest phase (lights-on) when mice consume less food (9–10 kJ) was normal between genotypes. Body weight and composition of AgRP-MC3R and LoxTBMc3r mice was similar, suggesting compensatory mechanisms for reduced calorie intake. Remarkably, AgRP-MC3R mice continued to consume less food during re-feeding after fasting and timed-restricted feeding. MC3Rs expressed on AgRP+ve neurons appear to exert a strong inhibitory signal on hypothalamic networks governing feeding behavior.
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影响因子：
3.5
作者：
Anderson EJ;Çakir I;Carrington SJ;Cone RD;Ghamari-Langroudi M;Gillyard T;Gimenez LE;Litt MJ
通讯作者：
Litt MJ
影响因子：
8.1
作者：
Lam BYH;Cimino I;Polex-Wolf J;Nicole Kohnke S;Rimmington D;Iyemere V;Heeley N;Cossetti C;Schulte R;Saraiva LR;Logan DW;Blouet C;O'Rahilly S;Coll AP;Yeo GSH
通讯作者：
Yeo GSH
影响因子：
7.7
作者：
Henry FE;Sugino K;Tozer A;Branco T;Sternson SM
通讯作者：
Sternson SM
影响因子：
25
作者：
Dietrich, Marcelo O.;Bober, Jeremy;Horvath, Tamas L.
通讯作者：
Horvath, Tamas L.
DOI：
10.1152/ajpregu.2000.279.1.r47
发表时间：
2000-07-01
影响因子：
2.8
作者：
Hagan, MM;Rushing, PA;Seeley, RJ
通讯作者：
Seeley, RJ