Dual-Responsive Controlled Drug Delivery Based on Ionically Assembled Nanoparticles
Dual-Responsive Controlled Drug Delivery Based on Ionically Assembled Nanoparticles
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基于离子组装纳米颗粒的双响应控制药物递送
DOI：
10.1021/la3016436
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发表时间：
2012-06-26
期刊：
影响因子：
3.9
通讯作者：
Lu, Qinghua
中科院分区：
文献类型：
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作者：
Cui, Wei;Lu, Xuemin;Lu, Qinghua
聚（离子液体-co-N-异丙基丙烯酰胺）与脱氧胆酸通过静电相互作用形成离子组装纳米粒子（INPs）。通过1H-1 NMR、FTIR、TEM、DLS等表征手段对INPs的结构和性能进行了研究，结果表明，INPs具有pH响应性脱氧胆酸（pK（a）= 6.2）和温度响应性N-异丙基丙烯酰胺（N-isopropylacrylamide）的双重响应特性。以阿霉素（DOX）为模型药物，研究了其作为药物载体的潜在应用。在较低的pH（pH 5.2）和较高的温度（高于37 ° C）下，由于质子化DA从INPs离开和在病理条件下较低的LCST（较低临界溶液温度），发生INPs的结构崩溃以及DOX的释放。结果表明，在pH 5.2，43 ℃下，80%的DOX分子在48 h内从INPs中释放，但在37 ℃和pH 7.4下，48 h内仅释放30%的药物。此外，载药INPs对细胞生长表现出抑制作用。
Ionically assembled nanoparticles (INPs) have been formed from poly(ionic liquid-co-N-isopropylacrylamide) with deoxycholic acid through electrostatic interaction. The structure and properties of the INPs were investigated by using H-1 NMR, Fourier transform infrared (FTIR), transmission electron microscopy (TEM), dynamic light scattering (DLS), and so on. Due to pH-responsive deoxycholic acid (pK(a) = 6.2) and thermo responsive N-isopropylacrylamide included in the ionic complex, the INPs exhibit highly pH and thermal dual-responsive properties. The potential practical applications as drug delivery carriers were demonstrated using doxorubicin (DOX) as a model drug. With a lower pH (pH 5.2) and higher temperature (above 37 degrees C), structural collapse of the INPs occurred as well as release of DOX owing to protonated DA departure from the INPs and a lower LCST (lower critical solution temperature) at the pathological conditions. The result shows that 80% of DOX molecules were released from INPs within 48 h at pH 5.2, 43 degrees C, but only 30% of the drug was released within 48 h at 37 degrees C and pH 7.4. Moreover, drug-loaded INPs exhibit an inhibitory effect on cell growth.