Metallic nanocrystallites-incorporated ordered mesoporous carbon as labels for a sensitive simultaneous multianalyte electrochemical immunoassay.
Metallic nanocrystallites-incorporated ordered mesoporous carbon as labels for a sensitive simultaneous multianalyte electrochemical immunoassay.
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DOI：
10.1016/j.bios.2015.05.046
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发表时间：
2015-11
影响因子：
12.6
通讯作者：
Yishan Fang;Xinjian Huang;Q. Zeng;Lishi Wang
中科院分区：
文献类型：
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作者：
Yishan Fang;Xinjian Huang;Q. Zeng;Lishi Wang
这项工作报告了一种简便，新颖的多分析物电化学免疫分析法，用于同时检测甲胎蛋白（AFP）和人表皮生长因子受体2型（HER-2），使用含金属的纳米材料限制在有序的介孔碳基质（OMC-M）作为标签。采用简单、经济、绿色的制备方法，以甲阶酚醛树脂为碳源，金属硝酸盐为金属源，制备了分散均匀的金属纳米复合材料。由于OMC纳米材料上负载了大量的金属纳米粒子，极大地放大了检测信号，且碳纳米管-壳聚糖良好的生物相容性使其保持了良好的稳定性，适用于生物免疫分析。在优化的实验条件下，该方法具有较高的灵敏度和选择性，AFP和HER-2在0.001 ~ 150 ng/mL范围内呈良好的线性关系，检测下限分别为0.6 pg/mL和0.35 pg/mL（S/N=3）。该免疫传感器具有方便、廉价、快速、特异性好、稳定性和重现性好等优点。该方法用于真实的人血清中AFP和HER-2的检测，结果令人满意，表明该方法可用于AFP和HER-2等肿瘤标志物的临床检测。
This work reports on a facile, novel multianalyte electrochemical immunoassay for simultaneous detection of a-fetoprotein (AFP) and human epidermal growth factor receptor type-2 (HER-2) using metal-containing nanomaterials confined in the ordered mesoporous carbon matrix (OMC-M) as labels. Well-dispersed uniform metallic nanocrystallites incorporated OMC materials were fabricated through a simple, economical, and green preparative strategy toward phenolic resol as a carbon source and metal nitrate as metal sources. The large amount of metallic nanocrystallites loading on the OMC nanomaterials, greatly amplified the detection signals, and the good biocompatibility of carbon nanotubes–chitosan retained excellent stability for the sandwich-type immunoassay. Under optimal experimental conditions, the proposed immunoassay exhibited high sensitivity and selectivity for the detection of analytes, providing a better linear response range from 0.001 to 150 ng/mL for AFP and for HER-2, with a lower limit of detectionof 0.6 pg/mL and 0.35 pg/mL (S/N=3), respectively. The immunosensor exhibited convenience, low cost, rapidity, good specificity, acceptable stability and reproducibility. Moreover, satisfactory results were obtained for the determination of AFP and HER-2 in real human serum samples, indicating that the developed immunoassay has the potential to find application in clinical detection of AFP and HER-2 and other tumor markers as an alternative approach.