Factors related to contraction and mechanical strength of collagen gels seeded with canine endotenon cells
Factors related to contraction and mechanical strength of collagen gels seeded with canine endotenon cells
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DOI：
10.1002/jbm.b.30757
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发表时间：
2007-08-01
影响因子：
3.4
通讯作者：
Amadio, Peter C.
中科院分区：
文献类型：
--
作者：
Chen, Meng-Yi;Sun, Yulong;Amadio, Peter C.
成纤维细胞可以将水合胶原晶格构建成组织样结构，该结构受初始培养条件的影响很大。本研究的目的是研究细胞浓度和胶原蛋白浓度对所得到的腱内源性成纤维细胞接种的胶原蛋白网格的收缩动力学和机械性能的影响。实验旨在评价细胞浓度（0、0.25、0.5和1.0 x 10（6）个细胞/mL）和胶原蛋白浓度（0.5、1.0、1.5和2.0 mg/mL）的影响。记录胶原晶格收缩42天，之后对晶格进行机械测试。高初始细胞浓度接种的胶原晶格比低初始细胞浓度接种的胶原晶格具有更短的收缩滞后期（p < 0.01），并且表现出更高的极限应力（p < 0.01）和瞬时模量和平衡模量（p < 0.01）。低胶原初始浓度培养的胶原网格收缩滞后期较短，瞬时模量和平衡模量均高于高胶原初始浓度培养的胶原网格（P < 0.01）。胶原初始浓度为0.5mg/mL时，胶原晶格的极限应力最大（P < 0.03）。(C)2007 Wiley Periodicals，Inc.
Fibroblasts can construct a hydrated collagen lattice to a tissue-like structure that is greatly influenced by initial culture conditions. The purpose of this study was to investigate the effects of cell concentration and collagen concentration on the contraction kinetics and mechanical properties of resultant endotenon-derived fibroblast-seeded collagen lattice. The experiment was designed to evaluate the effect of cell concentration (0, 0.25, 0.5, and 1.0 x 10(6) cells/mL) and coliagen concentration (0.5, 1.0, 1.5, and 2.0 mg/mL). Collagen lattice contraction was recorded for 42 days, after which time the lattices were mechanically tested. The collagen lattices seeded with higher initial cell concentration had a shorter contraction lag phase (p < 0.01), and exhibited a higher ultimate stress (p < 0.01) and instantaneous and equilibrium modulus (p < 0.01) than those seeded with a lower initial cell concentration. The coliagen lattices cultured with a lower initial coliagen concentration also had a shorter contraction lag phase, and exhibited greater instantaneous and equilibrium modulus (P < 0.01) than those cultured with higher initial collagen concentration. The collagen lattices of initial 0.5 mg/mL collagen concentration had the highest value of ultimate stress (P < 0.03). (C) 2007 Wiley Periodicals, Inc.