Harnessing phytochrome's glowing potential
Harnessing phytochrome's glowing potential
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DOI：
10.1073/pnas.0407645101
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发表时间：
2004-12-14
影响因子：
11.1
通讯作者：
Lagarias, JC
中科院分区：
文献类型：
--
作者：
Fischer, AJ;Lagarias, JC
对一种蓝藻光敏色素进行了定向进化，通过重塑其线性四吡咯假基的化学环境来阐明其光感觉活动的结构基础。除了确定对维持光敏色素天然光谱特性至关重要的载脂蛋白的一个小区域外，我们的研究还揭示了一个酪氨酸到组氨酸的突变，该突变将光敏色素转化为强烈的红色荧光胆道蛋白。这种酪氨酸在光敏色素超家族的所有成员中都是保守的，暗示直接参与光敏色素的初级光过程。荧光光敏色素突变体也很有希望将目前的遗传编码荧光蛋白扩展到近红外。
Directed evolution of a cyanobacterial phytochrome was undertaken to elucidate the structural basis of its light sensory activity by remodeling the chemical environment of its linear tetrapyrrole prosthetic group. In addition to identifying a small region of the apoprotein critical for maintaining phytochrome's native spectroscopic properties, our studies revealed a tyrosine-to-histidine mutation that transformed phytochrome into an intensely red fluorescent biliprotein. This tyrosine is conserved in all members of the phytochrome superfamily, implicating direct participation in the primary photoprocess of phytochromes. Fluorescent phytochrome mutants also hold great promise to expand the present repertoire of genetically encoded fluorescent proteins into the near infrared.