Chemical composition and in vitro antioxidative activity of a lemon balm (Melissa officinalis L.) extract
Chemical composition and in vitro antioxidative activity of a lemon balm (Melissa officinalis L.) extract
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DOI：
10.1016/j.lwt.2007.03.007
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发表时间：
2008-01-01
影响因子：
6
通讯作者：
Hiltunen, Raimo
中科院分区：
文献类型：
--
作者：
Dastmalchi, Keyvan;Dorman, H. T. Damien;Hiltunen, Raimo
香蜂草（Melissa officinalis L.）用450 ml/l乙醇水溶液通过中压液-固萃取进行萃取。采用Folin-Ciocalteu试剂法测定提取物中总酚含量，并采用高效液相色谱-光电二极管阵列检测器进行定性定量分析。香蜂草提取物中含有羟基肉桂酸衍生物和黄酮类化合物，根据色谱行为和光谱特征鉴定了咖啡酸、间香豆酸、圣草酚-7-O-葡萄糖苷、柚皮苷、橙皮苷、迷迭香酸、柚皮素、橙皮素。还研究了提取物在铁（III）还原、铁（II）螯合、1，1-二苯基-2-苦肼基、2，2 '-连氮双（3-乙基苯并噻唑啉-6-磺酸盐）、超氧阴离子和一氧化氮自由基清除以及β-胡萝卜素-亚油酸漂白试验抑制中的潜在体外抗氧化特性。该提取物在所有试验中均表现出抗氧化活性。然而，除了在β-胡萝卜素-亚油酸漂白试验中，其活性上级没食子酸和咖啡酸的活性，并且在统计学上与槲皮素和BHA无区别外，其不是Lis potent Lis阳性对照。异常高的抗氧化活性和该测定具有生物学相关性的事实证明了在氧化应激的离体和体内模型中进一步研究香蜂草提取物。(C)2007年，瑞士食品科学与技术协会。由爱思唯尔有限公司出版。保留所有权利。
The leaf material of lemon balm (Melissa officinalis L.) was extracted with 450 ml/l aqueous ethanol by medium pressure liquid-solid extraction. The total phenolic content of the extract was estimated as gallic acid equivalents by Folin-Ciocalteu reagent method and a qualitative-quantitative compositional analysis was carried out using high performance liquid chromatography Coupled with photodiode array detection. The lemon balm extract contained hydroxycinnamic acid derivatives and flavonoids with caffeic acid, m- coumaric acid, eriodictyol-7-O-glucoside, naringin, hesperidin, rosmarinic Licid, naringenin, hesperetin being identified based on their chromatographic behaviour and spectral characteristics. The extract was also investigated for potential in vitro antioxidant properties in iron(Ill) reduction, iron(II) chelation, 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonate), superoxide anion and nitric oxide free-radical scavenging, and inhibition of beta-carotene-linoleic acid bleaching assays. The extract demonstrated antioxidant activity in all the assays. However, it was not Lis potent Lis the positive controls except in the beta-carotene-linoleic acid bleaching assay, where its activity was superior to that of gallic and caffeic acids and statistically indistinguishable from quercetin and BHA. The exceptionally high antioxidant activity and the fact that this assay is of biological relevance warrants further investigation of lemon balm extract in ex vivo and in vivo models of oxidative stress. (C) 2007 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.