INTERFERON ACTION - CYTOPLASMIC AND NUCLEAR-LOCALIZATION OF THE INTERFERON-INDUCIBLE 52-KD PROTEIN THAT IS ENCODED BY THE IFI 202 GENE FROM THE GENE 200 CLUSTER
INTERFERON ACTION - CYTOPLASMIC AND NUCLEAR-LOCALIZATION OF THE INTERFERON-INDUCIBLE 52-KD PROTEIN THAT IS ENCODED BY THE IFI 202 GENE FROM THE GENE 200 CLUSTER
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DOI：
10.1089/jir.1993.13.43
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发表时间：
1993-02-01
期刊：
影响因子：
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通讯作者：
LENGYEL, P
中科院分区：
文献类型：
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作者：
CHOUBEY, D;LENGYEL, P
最近，我们报道了一个干扰素（IFN）诱导的鼠72 kD磷蛋白（204蛋白）由IFI 204基因从基因200簇编码的，位于核洛鲁斯和核植物中。现在，我们已经针对202蛋白提出了一个多克隆抗血清，该抗血清由同一基因duster的IFI 202基因编码，其区域与204蛋白的区域是同源的。使用抗血清，我们确定202蛋白是一种52 kD的磷蛋白，在用IFN-α处理后，可以将来自各种鼠系的细胞中的细胞水平提高至16倍。涉及细胞裂解物和间接免疫荧光显微镜的实验表明，202蛋白位于细胞质和细胞核中。用IFN处理细胞后，202蛋白首先积聚在细胞质，膜级分的表面上，并在长期处理IFN的处理后主要定位于细胞核中。在IFN处理的有丝分裂AKR细胞中，将202蛋白与染色体共定位。 202从IFN处理的AKR细胞中提取的蛋白质在体外结合了双链DNA。关于202个蛋白质功能的研究应通过完整的cDNA克隆的可用性以及细胞系的发现以及该蛋白质表达受损的小鼠的近交菌株来促进。
Recently, we reported that an interferon (IFN)-inducible, murine 72-kD phosphoprotein (the 204 protein) that is encoded by the Ifi 204 gene from the gene 200 cluster is localized in the nucleolus and the nucleoplasm. We have now raised a polyclonal antiserum against the 202 protein that is encoded by the Ifi 202 gene from the same gene duster and regions of which are homologous to those from the 204 protein. Using the antiserum, we established that the 202 protein is a 52-kD phosphoprotein whose level in cells from various murine lines can be increased up to 16-fold upon treatment with IFN-alpha. Experiments involving fractionation of cell lysates and indirect immunofluorescence microscopy of cultured cells revealed that the 202 protein was localized in the cytoplasm and the nucleus. Upon treatment of cells with IFN, the 202 protein first accumulated on the surface of a cytoplasmic, membranous fraction and after prolonged treatment with IFN it was localized mainly in the nucleus. In IFN-treated mitotic AKR cells, the 202 protein was colocalized with chromosomes. 202 protein extracted from IFN-treated AKR cells bound double-stranded DNA in vitro. Studies on 202 protein function should be facilitated by the availability of complete cDNA clones and the finding of cell lines and an inbred strain of mice in which the expression of this protein was impaired.