Drosophila Myosin-XX Functions as an Actin-Binding Protein To Facilitate the Interaction between Zyx102 and Actin
Drosophila Myosin-XX Functions as an Actin-Binding Protein To Facilitate the Interaction between Zyx102 and Actin
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果蝇肌球蛋白-XX 作为肌动蛋白结合蛋白发挥作用，促进 Zyx102 和肌动蛋白之间的相互作用。
DOI：
10.1021/bi401236c
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发表时间：
2014-01-21
期刊：
影响因子：
2.9
通讯作者：
Li, Xiang-dong
中科院分区：
文献类型：
--
作者：
Cao, Yang;White, Howard D.;Li, Xiang-dong
XX 类肌球蛋白是多种肌球蛋白超家族的成员，存在于昆虫和几种低等无脊椎动物中。 DmMyo20 是果蝇中的 XX 类肌球蛋白，由 dachs 编码，它是脂肪信号通路的关键下游组件，影响发育过程中的生长、亲和力和基因表达。序列分析表明，DmMyo20 包含一个独特的延伸，即运动结构域，后面是一个 IQ 基序和一个 C 末端尾部。进行生化研究。为了了解 DmMyo20 的特性，我们在杆状病毒/Sf9 系统中表达了几种包含运动结构域的 DmMyo20 截短构建体。我们发现 DmMyo20 的运动结构域既不具有 ATPase 活性，也不具有与 ATP 结合的能力，表明 DmMyo20 不具有分子运动功能。我们发现DmMyo20的运动结构域可以以不依赖于ATP的方式特异性结合肌动蛋白丝，并增强肌动蛋白丝和Zyx102（脂肪信号通路中DmMyo20的下游组件）之间的相互作用。这些结果表明，DmMyo20 在控制细胞分化的信号通路中充当支架蛋白，但不充当分子马达。
The class XX myosin is a member of the diverse myosin superfamily and exists in insects and several lower invertebrates. DmMyo20, the class XX myosin in Drosophila, is encoded by dachs, which functions as a crucial downstream component of the Fat signaling pathway, influencing growth, affinity, and gene expression during development. Sequence analysis shows that DmMyo20 contains a unique extension, the motor domain, followed by one IQ Motif, and a C-terminal tail. To investigate the biochemical. properties of DmMyo20, we expressed several DmMyo20 truncated constructs containing the motor domain in the baculovirus/Sf9 system. We found that the motor domain of DmMyo20 had neither ATPase activity nor the ability to bind to ATP, suggesting that DmMyo20 does not function as a molecular motor. We found that the motor domain of DmMyo20 could specifically bind to actin filaments in an ATP-independent manner and enhance the interaction between actin filaments and Zyx102, a downstream component of DmMyo20 in the Fat signaling pathway. These results suggest that DmMyo20 functions as a scaffold protein, but not as a molecular motor, in a signaling pathway controlling cell differentiation.