Detection of novel polyomaviruses in fruit bats in Indonesia.
Detection of novel polyomaviruses in fruit bats in Indonesia.
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在印度尼西亚果蝠中检测新型多瘤病毒。
DOI：
10.1007/s00705-015-2349-7
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发表时间：
2015
影响因子：
2.7
通讯作者：
Kobayashi S. et al.
中科院分区：
文献类型：
--
作者：
堀上 健斗;後藤 奈々;今井 裕;山田 雅保;Kobayashi S. et al.
蝙蝠是多种病毒病原体的重要天然宿主，包括多瘤病毒（PyV）。本研究的目的是：（i）确定哪些PyVs存在于印度尼西亚的蝙蝠和（ii）分析蝙蝠PyVs和其他已知PyVs之间的进化关系。使用广谱聚合酶链反应（PCR）为基础的检测，我们筛选PyV的DNA分离脾样本从82野生果蝠在印度尼西亚捕获。PyV基因组的片段中检测到10个82脾样品筛选，并获得8个全长病毒基因组序列，使用反向PCR方法。八个全病毒基因组序列的系统发育分析表明，BatPyVs形成两个不同的遗传簇内提出的genusOrthopolyomavirus是遗传上不同于以前描述的BatPyVs。有趣的是，一组BatPyVs与灵长类PyVs遗传相关，包括人类PyV9和棘状神经发育不良相关的PyV。这项研究确定了印度尼西亚果蝠中存在新的PyVs，并提供了有关这些BatPyVs的遗传信息。
Bats are an important natural reservoir for a variety of viral pathogens, including polyomaviruses (PyVs). The aims of this study were: (i) to determine which PyVs are present in bats in Indonesia and (ii) to analyze the evolutionary relationships between bat PyVs and other known PyVs. Using broad-spectrum polymerase chain reaction (PCR)-based assays, we screened PyV DNA isolated from spleen samples from 82 wild fruit bats captured in Indonesia. Fragments of the PyV genome were detected in 10 of the 82 spleen samples screened, and eight full-length viral genome sequences were obtained using an inverse PCR method. A phylogenetic analysis of eight whole viral genome sequences showed that BatPyVs form two distinct genetic clusters within the proposed genusOrthopolyomavirusthat are genetically different from previously described BatPyVs. Interestingly, one group of BatPyVs is genetically related to the primate PyVs, including human PyV9 and trichodysplasia spinulosa-associated PyV. This study has identified the presence of novel PyVs in fruit bats in Indonesia and provides genetic information about these BatPyVs.