Control of the flagellation pattern in Helicobacter pylori by FlhF and FlhG.
Control of the flagellation pattern in Helicobacter pylori by FlhF and FlhG.
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FlhF 和 FlhG 对幽门螺杆菌鞭毛模式的控制。
DOI：
10.1128/jb.00110-23
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发表时间：
2023
影响因子：
3.2
通讯作者：
Hoover,TimothyR
中科院分区：
文献类型：
--
作者：
Gibson,KatherineH;Botting,JackM;Al-Otaibi,Natalie;Maitre,Kriti;Bergeron,Julien;Starai,VincentJ;Hoover,TimothyR
在许多极鞭毛细菌中，FlhF和FlhG分别控制着鞭毛的位置和数量。FlhF和FlhG在具有多极鞭毛的细菌（如幽门螺杆菌）中的作用尚不清楚。DeletingflhGinH。幽门使鞭毛的分布模式从大多数细胞大约有四个鞭毛转变为鞭毛数量分布更宽、更均匀。据报道，在其他细菌中，删除flhfinh。幽门导致运动能力降低，鞭毛减退，鞭毛不正确定位到非极性部位。h的运动变体。幽门螺杆菌（pylori∆flh）突变体中鞭毛正确定位到细胞极的比例较高，但我们无法确定导致鞭毛向细胞极定位增加的遗传决定因素。然而，一种可运动的变体比ΔflhFparental菌株产生更多的鞭毛，这显然是由于误义突变infliF（编码MS环蛋白），它将Asn-255变成了天冬氨酸。重组FliFN255D，而非重组野生型FliF，在体外形成了宽约50 nm的有序环状组装体，显示出MS环结构。我们从这些发现推断，在缺乏FlhF的情况下，flfn225dvariant比野生型flfn225dvariant更有效地形成MS环。幽门螺杆菌在人的胃中定植，可引起多种疾病，包括消化性溃疡疾病和胃癌。鞭毛幽门菌的运动，这是寄主定植所必需的。FlhG和FlhF控制许多细菌的鞭毛模式。我们在h中找到了它。在幽门螺旋杆菌中，FlhG确保细胞具有大致相等数量的鞭毛，FlhF是鞭毛组装和定位所必需的。FlhF的提出是为了促进FlhF组装到MS环中，MS环是鞭毛组装中最早形成的结构之一。我们发现了一个FliF变体，在没有FlhF的情况下组装MS环，这支持了FlhF在促进MS环组装中的作用。
FlhF and FlhG control the location and number of flagella, respectively, in many polar-flagellated bacteria. The roles of FlhF and FlhG are not well characterized in bacteria that have multiple polar flagella, such asHelicobacter pylori. DeletingflhGinH. pylorishifted the flagellation pattern where most cells had approximately four flagella to a wider and more even distribution in flagellar number. As reported in other bacteria, deletingflhFinH. pyloriresulted in reduced motility, hypoflagellation, and the improper localization of flagella to nonpolar sites. Motile variants ofH. pylori∆flhFmutants that had a higher proportion of flagella localizing correctly to the cell pole were isolated, but we were unable to identify the genetic determinants responsible for the increased localization of flagella to the cell pole. One motile variant though produced more flagella than the ΔflhFparental strain, which apparently resulted from a missense mutation infliF(encodes the MS ring protein), which changed Asn-255 to aspartate. Recombinant FliFN255D, but not recombinant wild-type FliF, formed ordered ring-like assembliesin vitrothat were ~50 nm wide and displayed the MS ring architecture. We infer from these findings that the FliFN225Dvariant forms the MS ring more effectivelyin vivoin the absence of FlhF than wild-type FliF.IMPORTANCEHelicobacter pyloricolonizes the human stomach where it can cause a variety of diseases, including peptic ulcer disease and gastric cancer.H. pyloriuses flagella for motility, which is required for host colonization. FlhG and FlhF control the flagellation patterns in many bacteria. We found that inH. pylori, FlhG ensures that cells have approximately equal number of flagella and FlhF is needed for flagellum assembly and localization. FlhF is proposed to facilitate the assembly of FliF into the MS ring, which is one of the earliest structures formed in flagellum assembly. We identified a FliF variant that assembles the MS ring in the absence of FlhF, which supports the proposed role of FlhF in facilitating MS ring assembly.