Correlation of Leukocyte Telomere Length Measurement Methods in Patients with Dyskeratosis Congenita and in Their Unaffected Relatives.
Correlation of Leukocyte Telomere Length Measurement Methods in Patients with Dyskeratosis Congenita and in Their Unaffected Relatives.
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DOI：
10.3390/ijms18081765
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发表时间：
2017-08-13
影响因子：
5.6
通讯作者：
Gadalla SM
中科院分区：
文献类型：
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作者：
Khincha PP;Dagnall CL;Hicks B;Jones K;Aviv A;Kimura M;Katki H;Aubert G;Giri N;Alter BP;Savage SA;Gadalla SM
在人类研究中，已经使用了几种方法来测量端粒长度(TL)。由于实验室技术和输出参数的不同，很难直接比较这些研究的结果。我们比较了三种最常用的方法，定量聚合酶链式反应(QPCR)，荧光原位杂交流式细胞仪(Flow FISH)和Southern印迹的TL测量(TLMS)，在一组端粒生物学疾病先天性角化不良(DC)患者及其未受影响的亲属(对照组)中。我们观察到DC患者及其未患病亲属的Southern印迹平均TL和Flow FISH总淋巴细胞TL之间有很强的相关性(R2分别为0.68和0.73)。定量聚合酶链式反应的平均热释光与Southern印迹法之间的相关性不大(DC患者的R2为0.54，未患病亲属为0.43)。当比较qPCR平均TL和Flow FISH总淋巴细胞TL时，DC患者和未患病亲属的R2分别为0.49和0.42。总之，三种广泛使用的TL分析方法(qPCR、Flow FISH和Southern印迹)之间的相关性强度显著不同。在为研究和临床研究选择TL测量方法时，需要仔细考虑。
Several methods have been employed to measure telomere length (TL) in human studies. It has been difficult to directly compare the results from these studies because of differences in the laboratory techniques and output parameters. We compared TL measurements (TLMs) by the three most commonly used methods, quantitative polymerase chain reaction (qPCR), flow cytometry with fluorescence in situ hybridization (flow FISH) and Southern blot, in a cohort of patients with the telomere biology disorder dyskeratosis congenita (DC) and in their unaffected relatives (controls). We observed a strong correlation between the Southern blot average TL and the flow FISH total lymphocyte TL in both the DC patients and their unaffected relatives (R2 of 0.68 and 0.73, respectively). The correlation between the qPCR average TL and that of the Southern blot method was modest (R2 of 0.54 in DC patients and of 0.43 in unaffected relatives). Similar results were noted when comparing the qPCR average TL and the flow FISH total lymphocyte TL (R2 of 0.49 in DC patients and of 0.42 in unaffected relatives). In conclusion, the strengths of the correlations between the three widely used TL assays (qPCR, flow FISH, and Southern blot) were significantly different. Careful consideration is warranted when selecting the method of TL measurement for research and for clinical studies.
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影响因子：
4.5
作者：
Stone RC;Horvath K;Kark JD;Susser E;Tishkoff SA;Aviv A
通讯作者：
Aviv A
影响因子：
4
作者：
Aston KI;Hunt SC;Susser E;Kimura M;Factor-Litvak P;Carrell D;Aviv A
通讯作者：
Aviv A
DOI：
10.1016/j.mrfmmm.2011.04.003
发表时间：
2012-02-01
影响因子：
2.3
作者：
Aubert, Geraldine;Hills, Mark;Lansdorp, Peter M.
通讯作者：
Lansdorp, Peter M.
影响因子：
4.5
作者：
Aubert G;Baerlocher GM;Vulto I;Poon SS;Lansdorp PM
通讯作者：
Lansdorp PM
DOI：
10.1182/asheducation-2011.1.480
发表时间：
2011-12-01
期刊：
HEMATOLOGY-AMERICAN SOCIETY HEMATOLOGY EDUCATION PROGRAM
影响因子：
--
作者：
Dokal, Inderjeet
通讯作者：
Dokal, Inderjeet