DIRECTIONALITY AND POLARITY IN RECA PROTEIN-PROMOTED BRANCH MIGRATION
DIRECTIONALITY AND POLARITY IN RECA PROTEIN-PROMOTED BRANCH MIGRATION
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DOI：
10.1073/pnas.78.10.6018
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
LEHMAN, IR
中科院分区：
文献类型：
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作者：
COX, MM;LEHMAN, IR
大肠杆菌的recA蛋白促进全长线性双链和单链环状噬菌体.vphi.X174 DNA分子之间的完全链交换。EM对反应的分析证实，含有短的异双链区域的D环在线性双链的末端迅速形成，随后相对缓慢的分支迁移将D环转化为镍环双链(RFII)和移位的线性单链。线状链的异双链伸展和位移是一致的。线性双链内的异源序列阻止了分支的迁移，并导致D环的积累。虽然D环可以在线性双链的两端形成，但recA蛋白启动的分支迁移是在3‘’.fwdarw中唯一进行的。相对于线性双链的(-)链的5‘’方向。
The recA protein of Escherichia coli promotes the complete exchange of strands between full-length linear duplex and single-stranded circular phage .vphi.X174 DNA molecules. Analysis of the reaction by EM confirms that D loops containing short heteroduplex regions are rapidly formed at the ends of the linear duplex, followed by a relatively slow branch migration that converts the D loops to nicked circular duplexes (RFII) and displaced linear single strands. Heteroduplex extension and displacement of the linear strand are concerted. Heterologous sequences within the linear duplex halt branch migration and lead to the accumulation of D loops. Although D loops can be formed at either end of the linear duplex, recA protein-promoted branch migration proceeds uniquely in the 3'' .fwdarw. 5'' direction relative to the (-) strand of the linear duplex.