Spatial and Temporal Confined Photothermolysis of Cancer Cells Mediated by Hollow Gold Nanospheres Targeted to Epidermal Growth Factor Receptors.
Spatial and Temporal Confined Photothermolysis of Cancer Cells Mediated by Hollow Gold Nanospheres Targeted to Epidermal Growth Factor Receptors.
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针对表皮生长因子受体的空心金纳米球介导的癌细胞的空间和时间限制光热作用。
DOI：
10.1021/acsomega.8b00712
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发表时间：
2018
期刊：
影响因子：
4.1
通讯作者：
Li,Chun
中科院分区：
文献类型：
--
作者：
Ku,Geng;Huang,Qian;Wen,Xiaoxia;Ye,John;Piwnica-Worms,David;Li,Chun
迄今为止，一些研究已经调查了短脉冲激光在选择性肿瘤细胞破坏或其细胞杀伤机制中的潜在用途。计算机模拟的空间和时间分布的温度升高后，脉冲激光照射在一个无限小的点源估计，温度达到最高点后，一个单一的15 ns的激光脉冲在1035 ns。此外，温度升高被限制在亚微米的半径内，并在100 ns内返回到基线。为了研究15 ns激光脉冲对A431肿瘤细胞的影响，我们将中空金纳米球（HAuNS）与针对上皮生长因子受体的抗体（C225）结合。所得的纳米颗粒，C225-HAuNS，结合到细胞膜，内化，并分布在整个细胞质，与一些纳米颗粒运输到核膜附近。在使用光学显微镜安装到一个可调脉冲钛：蓝宝石激光器，观察到快速和广泛的损伤的肝癌细胞，而照射A431细胞预处理与非靶向HAuNS与脉冲激光或预处理C225-HAuNS与连续波激光诱导的细胞损伤最小。此外，在单个15 ns的激光脉冲后，C225-HAuNS处理的A431细胞与3 T3成纤维细胞共培养显示出选择性破坏的迹象。因此，与连续波激光相比，短脉冲激光的照射对结合HAuNS的肿瘤细胞的破坏性最大，并且对周围环境产生的热量最少。短脉冲激光对癌细胞的这种作用模式（即，局限性光热疗法）可能在选择性肿瘤细胞破坏中具有潜在的应用。
To date, a few studies have investigated the potential use of a short-pulsed laser in selective tumor cell destruction or its mechanism of cell killing. Computer simulation of the spatial and temporal profiles of temperature elevation after pulsed laser irradiation on an infinitesimal point source estimated that the temperature reached its highest point at ∼35 ns after a single 15 ns laser pulse. Moreover, temperature elevation was confined to a radius of sub-micrometer and returned to baseline within 100 ns. To investigate the effect of 15 ns laser pulses on A431 tumor cells, we conjugated hollow gold nanospheres (HAuNSs) to an antibody (C225) directed at the epithelial growth factor receptor. The resulting nanoparticles, C225-HAuNSs, bound to the cell membrane, internalized, and distributed throughout the cytoplasm, with some nanoparticles transported to the vicinity of the nuclear membrane. On using an optical microscope mounted to a tunable pulsed Ti:sapphire laser, rapid and extensive damage of live cancer cells was observed, whereas irradiation of A431 cells pretreated with nontargeted HAuNSs with a pulsed laser or pretreated with C225-HAuNSs with a continuous-wave laser-induced minimal cellular damage. Furthermore, after a single 15 ns laser pulse, C225-HAuNS-treated A431 cells cocultured with 3T3 fibroblasts showed signs of selective destruction. Thus, compared with a continuous-wave laser, shots of a short-pulsed laser were the most damaging to tumor cells that bound HAuNSs and generated the least heat to the surrounding environment. This mode of action by a short-pulsed laser on cancer cells (i.e., confined photothermolysis) may have potential applications in selective tumor cell destruction.