Quantitative Analysis of Tumor in Bronchial Biopsy Specimens
Quantitative Analysis of Tumor in Bronchial Biopsy Specimens
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DOI：
10.1097/jto.0b013e3181ca12c4
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发表时间：
2010-04-01
影响因子：
20.4
通讯作者：
Kerr, Keith M.
中科院分区：
文献类型：
--
作者：
Coghlin, Caroline L.;Smith, Louise J.;Kerr, Keith M.
背景：肺癌患者的诊断性支气管活检样本可用于分子生物学分析，以帮助选择治疗方法并提供预后信息。一些人认为，绕过组织学检查，对支气管活检碎片进行直接分子分析可能是可行的。我们分析了一系列肺癌患者的 100 份支气管活检标本，以评估活检样本中肿瘤存在的频率和数量。方法：通过使用计算机辅助形态测定法测量组织切片中的肿瘤面积来评估支气管活检标本中肿瘤的比例。结果：只有 48% 的病例所有活检碎片都含有一些肿瘤。支气管镜检查获得的碎片中位数为 4；实际含有肿瘤的中位数为 3。对于所有病例，活检碎片中肿瘤的平均总表面积（占总样本面积的百分比）为 33.4%；中位面积28%。小细胞癌活检样本中的肿瘤数量（平均面积 46.5%，中位数 49%；p = 0.0006）多于所有其他非小细胞癌病例。结论：恶性支气管活检样本通常含有有限数量的原发癌。通常，一个或多个活检片段不包含肿瘤。这对于用于遗传分析的支气管活检样本的储存和使用具有重要意义。
Background: Diagnostic bronchial biopsy samples from lung cancer patients may be used for molecular biologic analyses to help select therapy and provide prognostic information. Some have suggested that direct molecular analysis of bronchial biopsy fragments may be feasible, bypassing histologic examination. We analyzed a series of 100 bronchial biopsy specimens in lung cancer patients to assess the frequency and quantity of tumor present in biopsy samples.Methods: The proportion of tumor in bronchial biopsy specimens was assessed by measuring the tumor area in histologic sections using computer-aided morphometry.Results: In only 48% of cases did all the biopsy fragments contain some tumor. The median number of fragments obtained at bronchoscopy was 4; median number actually containing tumor was 3. The mean total surface area of tumor (as a percentage of the total sample area) in biopsy fragments was, for all cases, 33.4%; median area 28%. Biopsies with small cell carcinoma had more tumor (mean area 46.5%, median 49%; p = 0.0006) than all other non-small cell carcinoma cases.Conclusion: Malignant bronchial biopsy samples frequently contain limited amounts of primary carcinoma. Often, one or more of the biopsy fragments will not contain tumor. This has important implications for the storage and use of bronchial biopsy samples for genetic analysis.