Caenorhabditis elegans expresses a functional phytochelatin synthase
Caenorhabditis elegans expresses a functional phytochelatin synthase
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DOI：
10.1046/j.1432-1327.2001.02293.x
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发表时间：
2001-07-01
期刊：
影响因子：
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通讯作者：
Degenkolb, T
中科院分区：
文献类型：
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作者：
Clemens, S;Schroeder, JI;Degenkolb, T
植物螯合素（小分子金属结合谷胱甘肽衍生肽）的形成是植物对有毒金属暴露的充分研究的反应之一。在一些真菌和一些海洋硅藻中也检测到了植物螯合素。最近从拟南芥、小麦和粟酒裂殖酵母中克隆了编码植物螯合素合酶（PCS）的基因。令人惊讶的是，数据库搜索揭示了秀丽隐杆线虫基因组中存在同源基因，DDBJ/EMBL/GenBank 登录号为 10000。 266513。在这里，我们表明秀丽隐杆线虫确实表达编码功能性植物螯合素合酶的基因。 CePCS 补充了粟酒裂殖酵母 PCS 敲除菌株的 Cd2+ 敏感性，并赋予这些细胞植物螯合素合酶活性。因此，植物螯合素也可能在某些动物的金属稳态中发挥作用。
The formation of phytochelatins, small metal-binding glutathione-derived peptides, is one of the well-studied responses of plants to toxic metal exposure. Phytochelatins have also been detected in some fungi and some marine diatoms. Genes encoding phytochelatin synthases (PCS) have recently been cloned from Arabidopsis, wheat and Schizosaccharomyces pombe. Surprisingly, database searches revealed the presence of a homologous gene in the Caenorhabditis elegans genome, DDBJ/EMBL/GenBank accession no. 266513. Here we show that C. elegans indeed expresses a gene coding for a functional phytochelatin synthase. CePCS complements the Cd2+ sensitivity of a Schizosaccharomyces pombe PCS knock-out strain and confers phytochelatin synthase activity to these cells. Thus, phytochelatins may play a role for metal homeostasis also in certain animals.