Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking
Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking
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DOI：
10.7554/elife.12058
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发表时间：
2015-11-21
期刊：
影响因子：
7.7
通讯作者：
Arshavsky, Vadim Y.
中科院分区：
文献类型：
--
作者：
Pearring, Jillian N.;Spencer, William J.;Arshavsky, Vadim Y.
感觉纤毛由一组精选的信号蛋白质组成，这些蛋白质检测环境刺激。这些分子是如何被运送到感觉纤毛的，以及它们是否相互依赖进行特定的运输仍然知之甚少。在这里，我们研究了视觉色素视紫红质是否对将其他信号蛋白传递到光感受器细胞的感觉纤毛(外段)至关重要。视紫红质是最丰富的外节蛋白，其适当的运输对于这个细胞器的形成是必不可少的，这表明这种依赖可能存在。事实上，我们证明了在光感受器中产生cGMP第二信使的鸟苷环化酶-1需要视紫红质来维持细胞内的稳定性和外节段的传递。我们通过证明鸟苷环化酶-1是一种新的视紫红质结合蛋白来解释这种依赖性。这些发现扩大了视紫红质在视觉中的作用，从作为视觉色素和主要的外部片段构建块，到指导另一个关键信号蛋白的运输。
Sensory cilia are populated by a select group of signaling proteins that detect environmental stimuli. How these molecules are delivered to the sensory cilium and whether they rely on one another for specific transport remains poorly understood. Here, we investigated whether the visual pigment, rhodopsin, is critical for delivering other signaling proteins to the sensory cilium of photoreceptor cells, the outer segment. Rhodopsin is the most abundant outer segment protein and its proper transport is essential for formation of this organelle, suggesting that such a dependency might exist. Indeed, we demonstrated that guanylate cyclase-1, producing the cGMP second messenger in photoreceptors, requires rhodopsin for intracellular stability and outer segment delivery. We elucidated this dependency by showing that guanylate cyclase-1 is a novel rhodopsin-binding protein. These findings expand rhodopsin's role in vision from being a visual pigment and major outer segment building block to directing trafficking of another key signaling protein.