Detection of DNA in Aphid Honeydew: A Source of Error for Molecular Gut Content Analysis?
Detection of DNA in Aphid Honeydew: A Source of Error for Molecular Gut Content Analysis?
复制标题
蚜虫蜜露中 DNA 的检测：分子肠道内容物分析的误差来源？
DOI：
10.2317/151130.1
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
J. Harwood
中科院分区：
文献类型：
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作者：
Hannah J. Penn;J. Harwood
摘要蚜虫（半翅目：蚜科）是全球范围内多种农作物的害虫，每年造成重大的产量损失。生物防治，利用天敌来抑制重点害虫物种，往往被纳入蚜虫管理实践。为了确保节肢动物捕食者消耗目标蚜虫，并最终对害虫种群动态产生影响，收集有关空间和时间消耗模式的信息非常重要。分子肠道内容物分析可用于评估捕食者和猎物之间的营养联系的强度，通过检测其肠道中物种特异性DNA（包括蚜虫DNA）的存在。然而，许多多面手捕食者很容易消耗蚜虫蜜露作为一个额外的营养资源以外的直接消费的猎物。这就提出了一个重要的问题，蚜虫蜜露是否是负责假阳性捕食事件被记录使用基于DNA的肠道内容物分析，蜜露中含有可检测到的蚜虫DNA的数量。为了评估这种潜在的错误来源，我们研究了三种蚜虫，豌豆蚜，麦长管蚜，禾谷缢管蚜，蚜虫DNA使用标准的肠道内容物分析方法的蜜露。经过广泛的测试，我们发现，蚜虫蜜露不包含任何可检测到的蚜虫DNA的任何物种，也没有蜜露抑制蚜虫DNA的扩增在阳性对照实验。这些数据表明，蚜虫捕食率以前报道的蜜露的直接消费没有影响。因此，这项研究表明，使用基于DNA的肠道内容物分析是一种可靠的方法来衡量蚜虫捕食在外地，即使捕食者暴露于和消费，蜜露。
Abstract Aphids (Hemiptera: Aphididae) are pests of a vast array of crops globally, contributing to significant yield loss annually. Biological control, utilizing natural enemies to suppress focal pest species, has often been integrated into aphid management practices. To ensure that arthropod predators are consuming the target aphid, and ultimately have an impact on pest population dynamics, it is important to gather information on consumption patterns in space and time. Molecular gut content analysis can be used to assess the strength of trophic linkages between predators and prey by detecting the presence of species-specific DNA (including aphid DNA) in their gut. However, many generalist predators readily consume aphid honeydew as an additional nutritive resource beyond direct consumption of prey. This raises the important question of whether aphid honeydew is responsible for false-positive predation events being recorded using DNA-based gut content analysis, should honeydew contain detectable quantities of aphid DNA. To evaluate this potential source of error, we examined honeydew of three species of aphid, Acyrthosiphon pisum, Sitobion avenae, and Rhopalosiphum padi, for aphid DNA using standard gut content analysis methods. After extensive testing, we revealed that aphid honeydew did not contain any detectable aphid DNA for any of the species, nor did honeydew inhibit the amplification of aphid DNA in positive control experiments. These data indicate that aphid predation rates previously reported are not influenced by direct consumption of honeydew. This study, therefore, shows that using DNA-based gut content analysis is a reliable method to measure aphid predation in the field, even when predators are exposed to and consume, honeydew.