The importance of fitting in: conformational preference of selenium 2' modifications in nucleosides and helical structures.
The importance of fitting in: conformational preference of selenium 2' modifications in nucleosides and helical structures.
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DOI：
10.1080/07391102.2014.880944
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发表时间：
2015
影响因子：
4.4
通讯作者：
Germann MW
中科院分区：
文献类型：
--
作者：
Thompson RA;Spring AM;Sheng J;Huang Z;Germann MW
硒蛋氨酸掺入在蛋白质的X射线结晶学中被证明是有用的，以获得相信息。在核酸中，将硒引入到不同的位置也有利于解决相问题，但它在2‘位的加入也显著地促进了晶体的形成。单核苷酸中的Se修饰表现出对2‘-Endo糖折叠的偏好，这与现有的晶体结构相冲突，在现有晶体结构中，双掺入的2’-Se修饰核苷酸仅以3‘-Endo构象存在。我们的工作提供了一个理论基础，为什么2‘-硒修饰促进结晶，尽管这种矛盾的行为。
Selenomethionine incorporation has proven useful in x-ray crystallography of proteins to obtain phase information. In nucleic acids, the introduction of selenium to different positions is beneficial for solving the phase problem as well, but its addition to the 2' position also significantly enhances crystal formation. The selenium modification in a single nucleotide shows a preference towards 2'-endo sugar puckering, which is in conflict with existing crystal structures where the duplex incorporated 2'-selenium modified nucleotide is exclusively found in a 3'-endo conformation. Our work provides a rationale why 2'-selenium modifications facilitate crystallization despite this contradictory behavior.