Lactate levels in the brain are elevated upon exposure to volatile anesthetics A microdialysis study
Lactate levels in the brain are elevated upon exposure to volatile anesthetics A microdialysis study
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DOI：
10.1016/j.neuint.2010.09.014
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发表时间：
2010-12-01
影响因子：
4.2
通讯作者：
Klein, Jochen
中科院分区：
文献类型：
--
作者：
Horn, Tobias;Klein, Jochen
麻醉剂具有明确的药理学靶点，但其对脑中能量代谢的影响知之甚少。在这项研究中，我们检查了不同麻醉剂对小鼠血液CSF和脑中细胞外乳酸和葡萄糖水平的影响。用微透析法监测清醒小鼠和用七种不同麻醉药麻醉期间脑中的细胞外能量代谢产物。结果表明，异氟醚麻醉可引起小鼠纹状体和海马细胞外乳酸水平的显著升高，（300-400%）丙酮酸水平也增加，而葡萄糖和谷氨酸水平不变。依赖性，并被其他气体麻醉剂（如氟烷和七氟烷）模仿，但不被静脉麻醉剂氯胺酮/甲苯噻嗪和水合氯醛引起的2-小鼠血液和脑中葡萄糖水平增加1倍，而乳酸水平仅为中度增加丙泊酚引起细胞外葡萄糖水平轻微增加，而戊巴比妥对乳酸或葡萄糖均无影响挥发性麻醉剂也使血液和CSF中乳酸水平增加2-3-进一步的实验表明，小鼠脑中异氟烷的乳酸盐形成不依赖于神经元脉冲流，也不涉及ATP-依赖性钾通道我们得出结论，挥发性麻醉剂而不是静脉麻醉剂引起特定剂量-在小鼠脑中细胞外乳酸水平的依赖性增加这种作用在没有缺血的情况下发生，不依赖于外周作用，反映在CSF乳酸水平的强烈增加（C）2010 Elsevier Ltd版权所有
Anesthetic agents have well-defined pharmacological targets but their effects on energy metabolism in the brain are poorly understood In this study we examined the effects of different anesthetics on extracellular lactate and glucose levels in blood CSF and brain of the mouse In vivo-microdialysis was used to monitor extracellular energy metabolites in the brain of awake mice and during anesthesia with seven different anesthetic drugs In separate groups lactate and glucose concentrations in blood and CSF were measured for each anesthetic We found that anesthesia with isoflurane caused a large increase of extracellular lactate levels in mouse striatum and hippocampus (300-400%) Pyruvate levels also increased while glucose and glutamate levels were unchanged This effect was dose-dependent and was mimicked by other gaseous anesthetics such as halothane and sevoflurane but not by Intravenous anesthetics Ketamine/xylazine and chloral hydrate caused 2-fold Increases of glucose levels in mouse blood and brain while lactate levels were only moderately Increased Propofol caused a minor increase of extracellular glucose levels while pentobarbital had no effect on either lactate or glucose Volatile anesthetics also increased lactate levels in blood and CSF by 2-3-fold but had no effect on plasma glucose Further experiments demonstrated that lactate formation by isoflurane in mouse brain was independent of neuronal impulse flow and did not involve ATP-dependent potassium channels We conclude that volatile anesthetics but not intravenous anesthetics cause a specific dose-dependent increase in extracellular lactate levels in mouse brain This effect occurs in the absence of ischemia is Independent of peripheral actions and is reflected in strongly Increased CSF lactate levels (C) 2010 Elsevier Ltd All rights reserved