BdorOBP69a is involved in the perception of the phenylpropanoid compound methyl eugenol in oriental fruit fly (Bactrocera dorsalis) males
BdorOBP69a is involved in the perception of the phenylpropanoid compound methyl eugenol in oriental fruit fly (Bactrocera dorsalis) males
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DOI：
10.1016/j.ibmb.2022.103801
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发表时间：
2022-08-01
影响因子：
3.8
通讯作者：
Jiang，Hongbo
中科院分区：
文献类型：
--
作者：
Chen，Xiaofeng;Yang，Hui;Jiang，Hongbo
东方实蝇（Bactrocera dorsalis）是一种危害450多种植物的毁灭性果树害虫，甲基丁香酚（ME）作为雄性引诱剂已被用于监测和根除。dorsalispopulations的70年，但其活动的分子基础仍然很大程度上不清楚。以前，BdorOBP 83 b和BdorOBP 56 f-2作为气味结合蛋白（OBP）被鉴定为负责ME感知。在这项研究中，ME诱导的表达谱和体外结合试验表明，BdorOBP 69 a也是响应ME产生的，并以强亲和力直接与ME结合（Kd= 9.54 μM）。通过CRISPR/Cas9诱变产生的BdorOBP 69 a −/−无效突变体对ME的触角电位和行为反应显著低于野生型对照。分子对接分析和定点突变表明，BdorOBP 69 a与ME的相互作用依赖于Leu 89和Phe 145残基，因此BdorOBP 69 a是参与B中ME感知的重要组分。dorsalis是一个很有前途的分子靶标，为开发新的雄性引诱剂。分子对接和结合分析数据也为未来OBP基因操作和ME化学工程提高雄性引诱剂效率提供了重要参考。
The oriental fruit fly (Bactrocera dorsalis) is a devastating fruit pest that infests more than 450 plant species.Methyl eugenol (ME) has been used as male attractant to monitor and eradicateB. dorsalispopulations for 70 years, but the molecular basis of its activity remains largely unclear. Previously, BdorOBP83b and BdorOBP56f-2 as odorant binding proteins (OBPs) were identified responsible for ME perception. In this study, ME-induced expression profiles andin vitrobinding assays revealed that BdorOBP69a is also produced in response to ME and binds directly to it with strong affinity (Kd= 9.54 μM).BdorOBP69a−/−null mutants generated by CRISPR/Cas9 mutagenesis showed significantly lower electroantennogram and behavioral responses to ME than wild-type controls. Molecular docking analysis followed by site-directed mutagenesis showed that residues Leu89 and Phe145 are essential for the interaction between BdorOBP69a and ME. BdorOBP69a is therefore an important component involved in the perception of ME inB. dorsalisand a promising molecular target for the development of new male attractants. The molecular docking and binding assay data also provide an important reference for future OBP gene manipulation and ME chemical engineering to improve the efficiency of male attractants.