Effect of Electromagnetic Pulse Exposure on Brain Micro Vascular Permeability in Rats
Effect of Electromagnetic Pulse Exposure on Brain Micro Vascular Permeability in Rats
复制标题
电磁脉冲暴露对大鼠脑微血管通透性的影响
DOI：
10.1016/s0895-3988(09)60055-6
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发表时间：
2009-06-01
影响因子：
3.5
通讯作者：
Guo, Guo-Zhen
中科院分区：
文献类型：
--
作者：
Ding, Gui-Rong;Li, Kang-Chu;Guo, Guo-Zhen
目的观察电磁脉冲（EMP）暴露对大鼠脑微血管通透性的影响。方法雄性SD大鼠全身暴露于200 kV/m的电磁脉冲（200脉冲或400脉冲，1 Hz）或假暴露于200 kV/m的电磁脉冲。分别于EMP暴露后0.5、1、3、6、12 h，以硝酸镧和内源性白蛋白为血管示踪剂，采用透射电镜和免疫组织化学方法检测脑微血管通透性。结果硝酸镧示踪剂在对照组大鼠脑内仅限于微血管腔内，未见硝酸镧或白蛋白示踪剂外渗。EMP照射后，硝酸镧离子到达紧密连接、基底膜和毛细血管周围组织。同样，在毛细血管周围组织中发现白蛋白免疫阳性染色。脑微血管通透性的改变是短暂的，微血管通透性在照射后1h即出现，3 h达高峰，12 h基本恢复。另外，400脉冲电磁脉冲辐照后微血管渗漏较200脉冲辐照后明显。结论200 kV/m电磁脉冲200和400脉冲（1 Hz）暴露可引起大鼠脑微血管通透性增加，且具有恢复性。
Objective To observe the effect of electromagnetic Pulse (EMP) exposure on cerebral micro vascular permeability in rats. Methods The whole-body of male Sprague-Dawley rats were exposed or sham exposed to 200 pulses or 400 Pulses (1 Hz) of EMP at 200 kV/m. At 0.5, 1, 3, 6, and 12 h after EMP exposure, the permeability of cerebral micro vascular was detected by transmission electron microscopy and immunohistochemistry using lanthanum nitrate and endogenous albumin as vascular tracers, respectively. Results The lanthanum nitrate tracer was limited to the micro vascular lumen with no lanthanum nitrate or albumin tracer extravasation in control rat brain. After EMP exposure, the lanthanum nitrate ions reached the tight junction, basal lamina and pericapillary tissue. Similarly, the albumin immunopositive staining was identified in pericapillary tissue. The changes in brain micro vascular permeability were transient, the leakage of micro vascular vessels appeared at I h, and reached its peak at 3 h, and nearly recovered at 12 h, after EMP exposure. In addition, the leakage of micro vascular was more obvious after exposure of EMP at 400 Pulses than after exposure of EMP at 200 pulses. Conclusion Exposure to 200 and 400 pulses (1 Hz) of EMP at 200 kV/m can increase cerebral micro vascular permeability in rats, which is recoverable.