Induction of Human Natural Killer Cells Under Defined Conditions by Seamless Transition from Maintenance Culture of Pluripotent Stem Cells
Induction of Human Natural Killer Cells Under Defined Conditions by Seamless Transition from Maintenance Culture of Pluripotent Stem Cells
复制标题
通过多能干细胞维持培养的无缝过渡在规定条件下诱导人类自然杀伤细胞
DOI：
10.1007/978-1-0716-2160-8_4
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Saito Megumu K.
中科院分区：
文献类型：
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作者：
Niwa Akira;Saito Megumu K.
使用多能干细胞（PSC）作为自然杀伤细胞（NK细胞）的来源可以提高NK细胞相关疾病的发病机制分析和现成细胞药物生产的重现性。我们建立了一种在无血清和二维条件下从人PSC分化NK细胞的方法。我们的方法能够实现从PSC维持到NK细胞分化的无缝过渡，除了培养基交换和全培养传代外，不使用任何技术。
The use of pluripotent stem cells (PSCs) as a source of natural killer cells (NK cells) can improve reproducibility in the analysis of the pathogenesis of NK cell-associated diseases and in the production of off-the-shelf cellular medicines. We have developed a method for the differentiation of NK cells from human PSCs under serum-free and two-dimensional condition. Our method enables the seamless transition from maintenance of PSCs to differentiation of NK cells, without the use of any techniques other than medium exchange and whole culture passage.