Optimizing collagen transport through track-etched nanopores
Optimizing collagen transport through track-etched nanopores
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DOI：
10.1016/j.memsci.2008.04.066
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发表时间：
2008-08-15
影响因子：
9.5
通讯作者：
Ruberti, Jeffrey W.
中科院分区：
文献类型：
--
作者：
Bueno, Ericka M.;Ruberti, Jeffrey W.
通过纳米孔的聚合物传输是生物技术应用中分子分离和组织的潜在强大工具。我们的目标是通过模仿细胞介导的胶原组装来产生排列的胶原纤维：驱动溶液中的胶原单体穿过径迹蚀刻膜中排列的纳米孔，然后在孔出口处形成纤维。我们研究了 I 型去端肽胶原单体在中性冷溶液中通过聚碳酸酯径迹蚀刻膜的传输，该膜包含直径 80 nm、长 6 μm 的孔，面积分数为 2%。使用的水源浓度为 1.0、2.8 和 7.0 mg/ml，压差为 0、10 和 20。通过共价聚乙二醇结合使膜表面亲水化，以限制溶质-膜相互作用。由于这种缔合分子在半稀溶液中的复杂行为，胶原蛋白通过纳米孔的运输是一个非直观的过程。尽管如此，修改后的开孔模型提供了输运参数的合理预测。传输速率取决于浓度和压力，半稀溶液中的跨膜扩散率是稀溶液中的两倍，可能是通过协同扩散或聚合物夹带实现的。胶原蛋白运输最显着的增强是通过膜亲水化实现的。使用 2.8 mg 胶原蛋白/ml、10 in H2O 和亲水膜观察到最高单体通量（2.60 +/- 0.49 x 10(3) 分子 s(-1) 孔(-1)）运输的最高浓度（5.99 +/- 2.58 mg/ml）。 (c) 2008 Elsevier B.V. 保留所有权利。
Polymer transport through nanopores is a potentially powerful tool for separation and organization of molecules in biotechnology applications. Our goal is to produce aligned collagen fibrils by mimicking cell-mediated collagen assembly: driving collagen monomers in solution through the aligned nanopores in track-etched membranes followed by fibrillogenesis at the pore exit. We examined type I atelo-collagen monomer transport in neutral, cold solution through polycarbonate track-etched membranes comprising 80-nm diameter, 6-mu m long pores at 2% areal fraction. Source concentrations of 1.0, 2.8 and 7.0 mg/ml and pressure differentials of 0, 10 and 20 in H2O were used. Membrane surfaces were hydrophilized via covalent poly(ethylene glycol) binding to limit solute-membrane interaction. Collagen transport through the nanopores was a non-intuitive process due to the complex behavior of this associating molecule in semi-dilute solution. Nonetheless, a modified open pore model provided reasonable predictions of transport parameters. Transport rates were concentration- and pressure-dependent, with diffusivities across the membrane in semi-dilute solution 2-fold those in dilute solution, possibly via cooperative diffusion or polymer entrainment. The most significant enhancement of collagen transport was accomplished by membrane hydrophilization. The highest concentration transported (5.99 +/- 2.58 mg/ml) with the highest monomer flux (2.60 +/- 0.49 x 10(3) molecules s(-1) pore(-1)) was observed using 2.8 mg collagen/ml, 10 in H2O and hydrophilic membranes. (c) 2008 Elsevier B.V. All rights reserved.