Transcriptomics at Maize Embryo/Endosperm Interfaces Identifies a Transcriptionally Distinct Endosperm Subdomain Adjacent to the Embryo Scutellum[OPEN]
Transcriptomics at Maize Embryo/Endosperm Interfaces Identifies a Transcriptionally Distinct Endosperm Subdomain Adjacent to the Embryo Scutellum[OPEN]
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DOI：
10.1105/tpc.19.00756
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发表时间：
2020-04-01
期刊：
影响因子：
11.6
通讯作者：
Widiez, Thomas
中科院分区：
文献类型：
--
作者：
Doll, Nicolas M.;Just, Jeremy;Widiez, Thomas
胚盾片附近的胚乳细胞群体在运输功能上表现出转录组富集，并受胚发育的影响。种子是复杂的生物系统，包括三个遗传上不同的组织（胚、胚乳和母体组织）。然而，内核的复杂性使得它很难理解室间的相互作用，而不访问空间上准确的信息。在这里，我们利用大尺寸的玉米（玉米）内核的特点，在不同的胚胎/胚乳界面的组织全基因组表达谱。我们的分析确定了特定的转录组签名在两个接口组织相比，整个种子室：盾片糊粉层和新命名的胚乳相邻的盾片（EAS）。授粉后9 d左右出现EAS，持续时间约11 d，EAS仅限于与胚性盾片相邻的1 ~ 3层胚乳细胞中。其转录组富含编码转运蛋白的基因。胚胎特异性突变体中胚胎的缺失可以改变EAS标记基因的表达模式。在一些EAS细胞中检测到细胞死亡以及压碎的细胞壁的积累表明EAS是一个动态区，与胚接触的细胞层定期被消除，并且随着胚的生长，额外的胚乳细胞被招募到该动态区。
A population of endosperm cells adjacent to the embryo scutellum shows transcriptomic enrichment in transport functions and is influenced by embryo development.Seeds are complex biological systems comprising three genetically distinct tissues nested one inside another (embryo, endosperm, and maternal tissues). However, the complexity of the kernel makes it difficult to understand intercompartment interactions without access to spatially accurate information. Here, we took advantage of the large size of the maize (Zea mays) kernel to characterize genome-wide expression profiles of tissues at different embryo/endosperm interfaces. Our analysis identifies specific transcriptomic signatures in two interface tissues compared with whole seed compartments: the scutellar aleurone layer and the newly named endosperm adjacent to scutellum (EAS). The EAS, which appears around 9 d after pollination and persists for around 11 d, is confined to one to three endosperm cell layers adjacent to the embryonic scutellum. Its transcriptome is enriched in genes encoding transporters. The absence of the embryo in an embryo specific mutant can alter the expression pattern of EAS marker genes. The detection of cell death in some EAS cells together with an accumulation of crushed cell walls suggests that the EAS is a dynamic zone from which cell layers in contact with the embryo are regularly eliminated and to which additional endosperm cells are recruited as the embryo grows.