Understanding Microbial Arsenic-Mobilization in Multiple Aquifers: Insight from DNA and RNA Analyses.
Understanding Microbial Arsenic-Mobilization in Multiple Aquifers: Insight from DNA and RNA Analyses.
复制标题
了解微生物砷动员在多个含水层：从DNA和RNA分析的见解。
DOI：
10.1021/acs.est.1c04117
复制
发表时间：
2021-10
影响因子：
11.4
通讯作者：
W. Xiu;T. Ke;J. Lloyd;Jiaxing Shen;Naji M. Bassil;Hokyung Song;D. Polya;Yi Zhao;Huaming Guo
中科院分区：
文献类型：
--
作者：
W. Xiu;T. Ke;J. Lloyd;Jiaxing Shen;Naji M. Bassil;Hokyung Song;D. Polya;Yi Zhao;Huaming Guo
生物地球化学过程是地下水砷(As)富集的关键控制因素；然而，高溶解As和硫酸盐含水层中活跃的As生物地球化学过程和相关微生物却知之甚少。为了解决这一问题，利用16S rRNA基因(RDNA)和相关的16S rRNA测序技术，对河套盆地西部地下水-沉积物微生物区系中的地下水-沉积物地球化学、总微生物群落和活性微生物群落及其潜在功能进行了研究。底泥或地下水总微生物群落和活性微生物群落的相对丰度呈正相关。有趣的是，地下水活性微生物群落主要与氨氮和硫化物有关，而沉积物活性微生物群落与水可提取硝酸盐高度相关。沉淀物来源和地下水来源的活性微生物(rRNA/rDNA比率>；1)都注意到Fe(III)-还原剂(由氨氧化诱导)和As(V)-还原剂，强调通过Fe(III)和/或As(V)还原来动员As。此外，地下水和沉积物之间活跃的隐蔽硫循环也影响了AS的活化。沉积物来源的活性微生物可能参与了厌氧硫铁矿的氧化(由反硝化作用驱动)，而地下水来源的微生物与硫歧化和硫酸盐还原有关。本研究提供了一个扩展的全景概念模型，即活跃的As-N-S-Fe生物地球化学过程影响高溶解As和硫酸盐含水层中As的活化。
Biogeochemical processes critically control the groundwater arsenic (As) enrichment; however, the key active As-mobilizing biogeochemical processes and associated microbes in high dissolved As and sulfate aquifers are poorly understood. To address this issue, the groundwater-sediment geochemistry, total and active microbial communities, and their potential functions in the groundwater-sediment microbiota from the western Hetao basin were determined using 16S rRNA gene (rDNA) and associated 16S rRNA (rRNA) sequencing. The relative abundances of either sediment or groundwater total and active microbial communities were positively correlated. Interestingly, groundwater active microbial communities were mainly associated with ammonium and sulfide, while sediment active communities were highly related to water-extractable nitrate. Both sediment-sourced and groundwater-sourced active microorganisms (rRNA/rDNA ratios > 1) noted Fe(III)-reducers (induced by ammonium oxidation) and As(V)-reducers, emphasizing the As mobilization via Fe(III) and/or As(V) reduction. Moreover, active cryptic sulfur cycling between groundwater and sediments was implicated in affecting As mobilization. Sediment-sourced active microorganisms were potentially involved in anaerobic pyrite oxidation (driven by denitrification), while groundwater-sourced organisms were associated with sulfur disproportionation and sulfate reduction. This study provides an extended whole-picture concept model of active As-N-S-Fe biogeochemical processes affecting As mobilization in high dissolved As and sulfate aquifers.