1-Methyladenine-producing cell in starfish testis.
1-Methyladenine-producing cell in starfish testis.
复制标题
海星睾丸中产生 1-甲基腺嘌呤的细胞。
DOI：
10.1016/0014-4827(77)90241-5
复制
发表时间：
1977
影响因子：
3.7
通讯作者：
H. Kanatani
中科院分区：
文献类型：
--
作者：
J. Kubota;K. Nakao;H. Shirai;H. Kanatani
研究了1-甲基腺嘌呤（1-MeAde）在海星（Asterina pectinifera）睾丸中的产生位点。用促性腺物质（GSS）孵育精子发现精子不具有任何1- meade产生活性。除去大部分精子后，将睾丸成分分为睾丸壁部分和游离细胞部分。当比较它们产生1- meade的活性时，游离细胞组分的活性高于壁组分。游离细胞主要包括直径7 ~ 10 μm、胞质呈颗粒状的A细胞和直径4 ~ 5 μm、胞质透明的B细胞两种类型。纯化后的A细胞片段在GSS作用下产生1-MeAde，而B细胞片段不能产生该物质。因此，海星睾丸中产生1-MeAde的部位是A细胞。电镜观察显示，这些产生1- meade的细胞是非生发性和吞噬性的，位于睾丸的生发上皮。它们也存在于睾丸中心发育中的生殖细胞和精子中。
The site of production of 1-methyladenine (1-MeAde) in the testis of the starfish,Asterina pectinifera, was investigated. Incubation of spermatozoa with the gonad-stimulating substance (GSS) revealed that spermatozoa do not have any 1-MeAde-producing activity. After removing most of the spermatozoa, the testis components were divided into testicular wall fraction and free cell fraction. When their 1-MeAde-producing activity was compared, the free cell fraction showed higher activity than the wall fraction. The free cell fraction contained mainly two types of cells: A cells, 7–10 μm in diameter and with granular cytoplasm, and B cells, 4–5 μm and with clear cytoplasm. The purified A cell fraction was found to produce 1-MeAde under the influence of GSS, whereas the B cell fraction failed to produce the substance. It is therefore concluded that the site of production of 1-MeAde in starfish testes is the A cell. Electron microscopic observation of A-cells revealed that these 1-MeAde-producing cells are non-germinal and phagocytotic, and are located along the germinal epithelium of the testis. They are also found among the developing germ cells and spermatozoa in the center of the testis.