Temporal Properties of Flicker ERGs in Rabbit Model of Retinitis Pigmentosa.
Temporal Properties of Flicker ERGs in Rabbit Model of Retinitis Pigmentosa.
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兔色素性视网膜炎模型中闪烁 ERG 的时间特性。
DOI：
10.1167/iovs.17-22332
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Terasaki H.
中科院分区：
文献类型：
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作者：
Okado S;Ueno S;Kominami T;Nakanishi A;Inooka D;Sayo A;Kondo M;Terasaki H.
目的：我们确定重建的内视网膜对晚期遗传性视网膜变性兔眼闪烁视网膜电图（ERGs）的影响。方法：以18月龄野生型（WT）家兔6只和转基因视紫红质P347L （Tg）家兔4只为研究对象。频率为3.906 ~ 50.781 Hz的正弦刺激可诱发闪烁性脑电图。为了阻断ON和OFF视网膜通路，分别通过玻璃体内注射2-氨基-4-磷酸丁酸（APB）和6-氰基-7-硝基喹啉- 2,3 (1H, 4H)-二酮（CNQX）。分析了药物前后ERGs基本组分的幅值和相。通过相位和振幅矢量检测突触后APB （ON-）和CNQX （OFF-）敏感成分。结果：Tg家兔的时间特性与WT家兔不同，有其独特之处；在3.906 Hz时，振幅下降，但在15.625 Hz时，振幅增加了3.5倍以上。在Tg兔中，3.906 Hz处的振幅减小是由于ON和OFF分量相位差为180引起的。另一方面，在15.625 Hz时，Tg兔的振幅增加是由On和OFF分量的总和引起的，它们的相位差约为120。结论：Tg家兔视网膜神经元的重塑对其闪变ergg的时间特性有明显影响。对RP眼的闪烁ergg的评估必须仔细考虑当前的研究结果。
Purpose: We determined the effects of a remodeled inner retina on the flicker electroretinograms (ERGs) in a rabbit eye at an advanced stage of inherited retinal degeneration.Methods: Six wild-type (WT) and four rhodopsin P347L transgenic (Tg) rabbits were studied at 18 months of age. Flicker ERGs were elicited by sinusoidal stimuli at frequencies of 3.906 to 50.781 Hz. To block the ON and OFF retinal pathways, 2-amino-4-phosphonobutyric acid (APB), and 6-cyano-7-nitroquinoxaline-2, 3 (1H, 4H)-dione (CNQX), respectively, were injected intravitreally. The amplitudes and phases of the fundamental components of the pre-and postdrug ERGs were analyzed. The postsynaptic APB (ON-) and CNQX (OFF-) sensitive components were determined by examining the phases and amplitude vectors.Results: The temporal properties of the Tg rabbits were different from those of the WT rabbits and had unique features; at 3.906 Hz, the amplitude was depressed but it increased by more than 3.5-fold at 15.625 Hz. The reduction of the amplitude at 3.906 Hz in Tg rabbits was caused by a cancelation of the ON and OFF components by a phase difference of 180. On the other hand, an increase in the amplitude at 15.625 Hz in Tg rabbits was caused by the summation of the ON and OFF components, which had an approximate 120 phase difference.Conclusions: The temporal properties of the flicker ERGs of Tg rabbits were affected markedly by the remodeling of the retinal neurons. Evaluations of the flicker ERGs in RP eyes must be done with careful considerations of the current findings.