Intraembryonic, but not yolk sac hematopoietic precursors, isolated before circulation, provide long-term multilineage reconstitution
Intraembryonic, but not yolk sac hematopoietic precursors, isolated before circulation, provide long-term multilineage reconstitution
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DOI：
10.1016/s1074-7613(01)00190-x
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发表时间：
2001-09-01
期刊：
影响因子：
32.4
通讯作者：
Godin, I
中科院分区：
文献类型：
--
作者：
Cumano, A;Ferraz, JC;Godin, I
卵黄囊和胚胎内前体细胞对造血的相对贡献一直是一个有争议的问题。由于到目前为止，仅在循环开始后的胚胎组织中发现了重建活性，因此无法正式确定重建细胞的起源。在这里，我们在循环前分离卵黄囊和胚胎内脏胸膜，并在移植前将外植体保存在器官培养中。来自胚胎内部位的前体细胞在成年小鼠体内产生了超过6个月的多系造血后代。卵黄囊细胞只能提供短期的髓系重建。这一结果揭示了体内循环前胚胎组织的不同造血力，表明只有胚胎内来源的细胞才能在成人体内长期造血。
The relative contribution of yolk sac and intraembryonic precursors to hematopoiesis has been a matter of long-standing controversy. As reconstitution activity has so far only been found in embryonic tissues after the onset of circulation, the origin of reconstituting cells could not be formally established. Here, we separated yolk sac and intraembryonic splanchnopleura prior to circulation and maintained the explants in organ culture before transfer. Precursors derived from the intraembryonic site generated multilineage hematopoietic progeny in adult mice for more than 6 months. Yolk sac cells only provided myeloid short-term reconstitution. The results reveal a differential hematopoietic capacity of precirculation embryonic tissues in vivo, and indicate that the only cells capable of adult long-term hematopoiesis are of intraembryonic origin.