Comprehensive pathogen detection associated with four recurrent episodes of Kawasaki disease in a patient during a single year using next-generation sequencing.
Comprehensive pathogen detection associated with four recurrent episodes of Kawasaki disease in a patient during a single year using next-generation sequencing.
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DOI：
10.1099/jmmcr.0.005019
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Kuroda M
中科院分区：
文献类型：
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作者：
Hamada H;Sekizuka T;Oba K;Katano H;Kinumaki A;Terai M;Mizutani T;Kuroda M
川崎病（Kawasaki disease， KD）是儿童最常见的多系统血管炎。病原体可能与KD的发病有关。然而，关于这种疾病的报道普遍缺乏一致性。本病例为1岁男童，在一年内有4次复发性KD发作，我们使用新一代测序分析了他的血清和咽标本中的病原体读数。对比分析血清样品中鉴定的细菌读数发现，链球菌和嗜血杆菌等细菌与治疗前第一次和第四次发作有显著相关性（R2 = 0.9506）。在第一恢复期，链球菌数量明显减少（P < 0.0001）。通过对第四期咽标本的测序，发现与第四期血清有类似的相关性（R2 = 0.6633）。在这种情况下，链球菌可能与KD的发病有关。必须进行进一步的研究来评估微生物感染与KD发病机制的推定关联。
Kawasaki disease (KD) is the most common multisystem vasculitis in childhood. Pathogens can be associated with the onset of KD. However, a lack of consistency prevails among reports about this disease. For this case of a 1-year-old boy with four recurrent episodes of KD within a year, we analysed profiles of pathogen reads in his serum and pharynx specimens using next-generation sequencing. Comparative analysis of the identified bacterial reads from serum samples found significant correlation of bacteria such as Streptococcus and Haemophilus spp. with the first and fourth episodes (R2 = 0.9506) before treatment. In the first convalescent phase, the number of Streptococcus spp. was reduced remarkably (P < 0.0001). From sequencing of the pharynx specimen from the fourth episode, a similar correlation was found with serum from the fourth episode (R2 = 0.6633). In this case, Streptococcus spp. may have been associated with onset of KD. Further studies must be undertaken to evaluate the putative association of micro-organism infection with KD pathogenesis.