Gut Microbiome and Metabolome Were Altered and Strongly Associated With Platelet Count in Adult Patients With Primary Immune Thrombocytopenia
Gut Microbiome and Metabolome Were Altered and Strongly Associated With Platelet Count in Adult Patients With Primary Immune Thrombocytopenia
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原发性免疫性血小板减少症成年患者的肠道微生物组和代谢组发生改变，且与血小板计数密切相关
DOI：
10.3389/fmicb.2020.01550
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发表时间：
2020-07-08
影响因子：
5.2
通讯作者：
Qian, Wenbin
中科院分区：
文献类型：
--
作者：
Zhang, Xuewu;Gu, Silan;Qian, Wenbin
肠道微生物群与许多自身免疫性疾病的发病机制有关。这仍然是一个活跃的研究领域，因为肠道微生物群对原发性免疫性血小板减少症（ITP）的作用仍不清楚。在这项研究中，使用30名未经治疗的成人原发性ITP患者和29名健康对照（HC）的粪便样本来研究肠道微生物群落和代谢产物谱。我们的研究结果表明，粪便细菌，如布劳特氏菌，链球菌，乳酸杆菌是丰富的，而细菌，如拟杆菌是耗尽ITP患者。值得注意的是，粪便代谢物如脂肪酰和甘油磷脂富集，并与差异性肠道微生物群密切相关。此外，魏氏菌和咽峡炎链球菌或Cer（t18：0/16：0）、Cer（d18：1/17：0）和13-羟基十八烷酸的组合可提供ITP的良好诊断标志物。此外，血小板计数与肠道菌群改变（如S。心绞痛和肠道代谢产物如Cer（t18：0/16：0）。总之，与HC相比，ITP患者的肠道微生物群和代谢组均出现生态失调。几种ITP改变的肠道细菌和代谢物可以作为ITP的诊断生物标志物，并且与血小板计数高度相关，表明它们也可能在ITP发病机制中发挥作用。
Gut microbiota has been implicated in the pathogenesis of many autoimmune diseases. This is still an area of active research given that the role of gut microbiota on the primary immune thrombocytopenia (ITP) remains unclear. In this study, fecal samples of 30 untreated adult primary ITP patients and 29 healthy controls (HCs) were used to investigate the gut microbial community and metabolite profiles. Our results show that fecal bacteria such as Blautia, Streptococcus, and Lactobacillus are enriched, whereas bacteria such as Bacteroides are depleted in ITP patients. Notably, fecal metabolites such as fatty acyls and glycerophospholipids are enriched and strongly correlate with discrepant gut microbiota. Furthermore, combinations of Weissella and Streptococcus anginosus, or Cer (t18:0/16:0), Cer (d18:1/17:0), and 13-hydroxyoctadecanoic acid could provide good diagnostic markers for ITP. Moreover, a strong negative correlation was found between platelet count and altered gut microbiota such as S. anginosus and gut metabolites such as Cer (t18:0/16:0) in ITP. In conclusion, dysbiosis of both gut microbiota and metabolome develops in ITP patients compared to HCs. Several ITP-altered gut bacteria and metabolites can be diagnostic biomarkers for ITP, and are highly correlated with platelet count, suggesting that they may also play a role in ITP pathogenesis.