Increase of oligodendrocyte progenitor cells after spinal cord injury
Increase of oligodendrocyte progenitor cells after spinal cord injury
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DOI：
10.1002/jnr.1180
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发表时间：
2001-09-15
影响因子：
4.2
通讯作者：
Uyemura, K
中科院分区：
文献类型：
--
作者：
Ishii, K;Toda, M;Uyemura, K
脊髓损伤后少突胶质祖细胞的反应还不清楚。在这项研究中，我们研究了少突胶质细胞的反应后，挫伤脊髓损伤的成年大鼠免疫组织化学。通过用单克隆抗体（mAbs）A2 B5和O 4染色来鉴定OPCs。A2 B5、O 4阳性的OPCs和半乳糖苷阳性的少突胶质细胞在背后索病变中显著增加。溴脱氧尿苷（BrdU）掺入研究表明，大多数O 4-阳性细胞在病变与BrdU标记，表明这些OPCs增殖。与此相反，髓鞘碱性蛋白的表达减少，在病变与对照组相比，仅接受椎板切除术。从损伤的脊髓中，OPCs通过用mAb A2 B5免疫淘洗分离。与对照组和未手术动物相比，我们观察到损伤脊髓中的OPC数量增加。在培养几天后，来自病变的OPCs表达半乳糖苷。这些结果表明，OPCs在成年大鼠脊髓损伤后被诱导分化。J. Neurosci. Res. 65：500-507，2001. (C)2001 Wiley-Liss，Inc.
The reaction of oligodendrocyte progenitor cells (OPCs) after spinal cord injury (SCI) is poorly understood. In this study, we examined oligodendroglial reactions after contusion SCI in adult rats by immunohistochemistry. OPCs were identified by staining with monoclonal antibodies (mAbs) A2B5 and O4. Each of the A2B5-, O4-positive OPCs and galactocerebroside-positive oligodendrocytes dramatically increased in the lesion of the dorsal posterior funiculus. Bromodeoxyuridine (BrdU) incorporation studies showed that most O4-positive cells in the lesion were labeled with BrdU, suggesting that these OPCs were proliferative. In contrast, the expression of myelin basic protein was decreased in the lesion compared with controls that received laminectomy only. From the injured cord, OPCs were isolated by immunopanning with mAb A2B5. We observed an increased number of OPCs from the injured spinal cords compared with those isolated from controls and unoperated animals. After several days in culture, the OPCs from the lesion expressed galactocerebroside. These results suggest that OPCs are induced and can differentiate following SCI in the adult rat. J. Neurosci. Res. 65:500-507, 2001. (C) 2001 Wiley-Liss, Inc.