Crystallization and preliminary crystallographic analysis of DJ-1, a protein associated with male fertility and parkinsonism
Crystallization and preliminary crystallographic analysis of DJ-1, a protein associated with male fertility and parkinsonism
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DOI：
10.1107/s090744490301271x
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发表时间：
2003-08-01
影响因子：
2.2
通讯作者：
Inagaki, F
中科院分区：
文献类型：
--
作者：
Honbou, K;Suzuki, NN;Inagaki, F
DJ-1被鉴定为一种新的癌基因产物，可与活化的RAS协同转化小鼠NIH3T3细胞。DJ-1还与男性不育症和帕金森病有关。DJ-1用柠檬酸钠和HEPES在pH 7.5的条件下结晶。该晶体属于P3(1)或P3(2)空间群，晶胞参数a=75.04，c=74.88埃，一个不对称单元含有两个分子。强度数据集被收集到2.00埃分辨率。
DJ-1 was identified as a novel oncogene product that transformed mouse NIH3T3 cells in cooperation with activated Ras. DJ-1 was also correlated with male infertility and parkinsonism. DJ-1 was crystallized using sodium citrate and HEPES at pH 7.5. The crystal belongs to space group P3(1) or P3(2), with unit-cell parameters a = 75.04, c = 74.88 Angstrom and contains two molecules in an asymmetric unit. An intensity data set was collected to 2.00 Angstrom resolution.