Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the X-linked gene, Rhox5/Pem, at preimplantation stages
Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the X-linked gene, Rhox5/Pem, at preimplantation stages
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DOI：
10.1016/j.cub.2005.11.071
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发表时间：
2006-01-24
期刊：
影响因子：
9.2
通讯作者：
Okabe, M
中科院分区：
文献类型：
--
作者：
Kobayashi, S;Isotani, A;Okabe, M
哺乳动物雄性植入前胚胎比雌性胚胎发育得更快[1, 2]。使用增强型绿色荧光蛋白 (EGFP) 标记的 X 染色体来识别胚胎的性别，我们通过 DNA 微阵列比较了雄性和雌性小鼠囊胚之间的基因表达模式。我们检测到近 600 个具有统计显着性相关表达的基因；大多数差异为 2 倍或更少。在 11 个显示出大于 2.5 倍差异的基因中，有 4 个基因完全或几乎完全依赖于性别表达。两个基因（Dby 和 Eif2s3y）被定位到 Y 染色体并在雄性囊胚中表达。其余两个（Rhox5/Pem 和 Xist）被定位到 X 染色体，主要在雌性囊胚中表达。此外，Rhox5/Pem 主要由父系遗传的 X 染色体表达，表明早期表观遗传基因调控存在性别差异。
Mammalian male preimplantation embryos develop more quickly than females [1, 2]. Using enhanced green fluorescent protein (EGFP)-tagged X chromosomes to identify the sex of the embryos, we compared gene expression patterns between male and female mouse blastocysts by DNA microarray. We detected nearly 600 genes with statistically significant sex-linked expression; most differed by 2-fold or less. Of 11 genes showing greater than 2.5-fold differences, four were expressed exclusively or nearly exclusively sex dependently. Two genes (Dby and Eif2s3y) were mapped to the Y chromosome and were expressed in male blastocysts. The remaining two (Rhox5/Pem and Xist) were mapped to the X chromosome and were predominantly expressed in female blastocysts. Moreover, Rhox5/Pem was predominantly expressed from the paternally inherited X chromosome , indicating sex differences in early epigenetic gene regulation.