Molecular weights of mitochondrial and cytoplasmic aminoacyl-tRNA synthetases of beef liver and their complexes.
Molecular weights of mitochondrial and cytoplasmic aminoacyl-tRNA synthetases of beef liver and their complexes.
复制标题
牛肝线粒体和细胞质氨酰-tRNA合成酶及其复合物的分子量。
作者：
E. Walker;G. B. Treacy;P. Jeffrey
在真核生物中，含有5-9个氨酰-tRNA合成酶活性的多酶复合体经常被报道。在这项研究中，我们报道了在牛肝细胞质中存在一个包含至少16个活性的多酶复合体，这种复合体可以被匀浆破坏以产生更小的复合体和非复合体合成酶。对这些复合体的大小和组分活性以及所有20种游离合成酶的相对分子质量的测定表明，通常情况下确定的较小的复合体和自由活性来自较大的复合体，在均质过程中有明确的阶段。我们还发现，在牛肝线粒体中发现了类似的复合体，但不是完全相同的，并给出了16种线粒体合成酶的分子量。
In eukaryotes, multienzyme complexes containing five to nine aminoacyl-tRNA synthetase activities have frequently been reported. In this study, we report the existence, in bovine liver cytoplasm, of a multienzyme complex containing at least 16 activities which can be disrupted by homogenization to give rise to smaller complexes and noncomplexed synthetases. Determination of the size and component activity of these complexes and of the molecular weights of all 20 free synthetases suggests that the smaller complexes and free activities normally identified arise from the larger complex by well-defined stages during homogenization. We also show that similar, though not identical, complexes are found in bovine liver mitochondria and give the molecular weights of 16 mitochondrial synthetases.