The transcription factor TCFL5 responds to A-MYB to elaborate the male meiotic program in mice.
The transcription factor TCFL5 responds to A-MYB to elaborate the male meiotic program in mice.
复制标题
DOI：
10.1530/rep-22-0355
复制
发表时间：
2023-02-01
期刊：
影响因子：
--
通讯作者：
Özata DM
中科院分区：
文献类型：
--
作者：
Cecchini K;Biasini A;Yu T;Säflund M;Mou H;Arif A;Eghbali A;Colpan C;Gainetdinov I;de Rooij DG;Weng Z;Zamore PD;Özata DM
在雄性小鼠中，转录因子STRA8和MEISON启动减数分裂I.我们报道STRA8/MEISON激活转录因子A-MYB和TCFL5，它们在精原细胞进入减数分裂后共同重编程基因表达。TCFL5促进减数分裂、mRNA周转、miR-34/449产生、减数分裂退出和精子发生所需基因的转录。这种转录结构在恒河猴中是保守的，这表明TCFL5在胎盘哺乳动物的减数分裂和精子发生中起着核心作用。Tcfl5em1/em1突变体不育，精子发生在减数分裂的中期或后期停止。此外，Tcfl5+/em1突变体产生的精子活性更低。睾丸特异性转录因子TCFL5在粗线精母细胞中表达，与转录因子A-MYB协同调节减数分裂基因表达程序。
In male mice, the transcription factors STRA8 and MEISON initiate meiosis I. We report that STRA8/MEISON activates the transcription factors A-MYB and TCFL5, which together reprogram gene expression after spermatogonia enter into meiosis. TCFL5 promotes transcription of genes required for meiosis, mRNA turnover, miR-34/449 production, meiotic exit, and spermiogenesis. This transcriptional architecture is conserved in rhesus macaque, suggesting TCFL5 plays a central role in meiosis and spermiogenesis in placental mammals. Tcfl5em1/em1 mutants are sterile, and spermatogenesis arrests at the mid- or late-pachytene stage of meiosis. Moreover, Tcfl5+/em1 mutants produce fewer motile sperm. The testis-specific transcription factor, TCFL5, expressed in pachytene spermatocytes regulates the meiotic gene expression program in collaboration with the transcription factor A-MYB.