A novel highly acidic polysaccharide with inhibitory activity on calcification from the calcified scale "coccolith" of a coccolithophorid alga, Pleurochrysis haptonemofera.
A novel highly acidic polysaccharide with inhibitory activity on calcification from the calcified scale "coccolith" of a coccolithophorid alga, Pleurochrysis haptonemofera.
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DOI：
10.1016/j.bbrc.2007.04.078
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发表时间：
2007-06
影响因子：
3.1
通讯作者：
N. Ozaki;S. Sakuda;H. Nagasawa
中科院分区：
文献类型：
--
作者：
N. Ozaki;S. Sakuda;H. Nagasawa
颗石是由海洋单细胞颗石藻产生的具有种特异性微细结构的钙化鳞片，由碳酸钙（CaCO 3）晶体和少量有机基质组成。从一种球石藻的球石中分离得到一种新的多糖，命名为球石基质酸性多糖（CMAP）。通过化学分析和核磁共振光谱（包括COSY、TOCSY、HMQC和HMBC）确定CMAP的结构是一种由以下单元组成的多糖：→4）l-艾杜糖醛酸（α1→2）内消旋酒石酸（3→1）乙醛酸（1→.它有四个羧基，每个二糖单位，如观察到的另一种多糖PS-2的特点，以前在侧鞭金藻。CMAP对CaCO 3沉淀有较强的抑制作用。这些结果表明，CMAP作为一个调节器的钙化的颗石。
Coccolith, a calcified scale with species-specific fine structure produced by marine unicellular coccolithophorid algae, consists of calcium carbonate (CaCO3) crystals and a small amount of organic matrices. A novel polysaccharide named coccolith matrix acidic polysaccharide (CMAP) was isolated from the coccolith of a coccolithophorid alga, Pleurochrysis haptonemofera. The structure of CMAP was determined by chemical analysis and NMR spectroscopy including COSY, TOCSY, HMQC, and HMBC to be a polysaccharide composed of the following unit: →4) l-iduronic acid (α1→2) meso-tartaric acid (3→1) glyoxylic acid (1→. It has four carboxyl groups per a disaccharide unit as observed in another polysaccharide PS-2 characterized previously in Pleurochrysis carterae. CMAP showed a strong inhibitory activity on CaCO3precipitation. These results suggest that CMAP serves as a regulator in the calcification of the coccolith.