AZIDE-RESISTANT MUTANTS OF ESCHERICHIA-COLI ALTER THE SECA-PROTEIN, AN AZIDE-SENSITIVE COMPONENT OF THE PROTEIN EXPORT MACHINERY
AZIDE-RESISTANT MUTANTS OF ESCHERICHIA-COLI ALTER THE SECA-PROTEIN, AN AZIDE-SENSITIVE COMPONENT OF THE PROTEIN EXPORT MACHINERY
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DOI：
10.1073/pnas.87.21.8227
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发表时间：
1990-11-01
影响因子：
11.1
通讯作者：
JAROSIK, GP
中科院分区：
文献类型：
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作者：
OLIVER, DB;CABELLI, RJ;JAROSIK, GP
大肠杆菌azi突变体，其生长对毫摩尔浓度的叠氮化钠具有抗性，是最早的大肠杆菌之一。大肠杆菌突变株分离。遗传互补、作图和DNA序列分析现在表明，这些突变是secA基因的等位基因，secA基因是蛋白质跨E. coli质膜。我们已经发现叠氮化钠是体内蛋白质输出的一种非常快速和有效的抑制剂，并且azi突变体对这种抑制更有抵抗力。此外，SecA依赖的体外蛋白质转运和ATP酶活性被叠氮化钠抑制，并且从azi突变株制备的SecA蛋白对这种抑制更具抗性。这些研究指出了蛋白质输出的特异性抑制剂，如叠氮化钠，在促进蛋白质输出机制的单个组分的功能的解剖中的效用。
Escherichia coli azi mutants, whose growth is resistant to millimolar concentrations of sodium azide, were among the earliest E. coli mutants isolated. Genetic complementation, mapping, and DNA sequence analysis now show that these mutations are alleles of the secA gene, which is essential for protein export across the E. coli plasma membrane. We have found that sodium azide is an extremely rapid and potent inhibitor of protein export in vivo and that azi mutants are more resistant to such inhibition. Furthermore, SecA-dependent in vitro protein translocation and ATPase activities are inhibited by sodium azide, and SecA protein prepared from an azi mutant strain is more resistant to such inhibition. These studies point to the utility of specific inhibitors of protein export, such as sodium azide, in facilitating the dissection of the function of individual components of the protein export machinery.